XB-CLONE-3240005
Clone Name: IMAGE:7715928
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Gene:
mrtfa
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976284 [+]
cacctattcagtctgcactaggaaggctggaggatttcctagagagttcaactggcagcccactactacgggggaaccaagatggtcctgcttttatgcc
tcttattgatgacctccatagccagatgctgagcagcttggccatcttagatcacccaccctcacctatggacacatctgatctgcacttctcccctgta
ggagactccctaggattagacatttctgaacctcctttggatggtatggactggcttgagctgtctgagcctccggctatgaatctgactccactcagca
cagctacccccagtgtcttttccactgatttcttggacagccatgatctgcagttacactgggattcctgtttataacctagctattagttggggattaa
gaaacaaatgtggatgaagaacgagttcagcacttttcctggcccctttaggaaattccttctttgccccccttgagtggtacttacggaaactgaaaga
aaatgctcagttcagggtgccaaattgtagggaatccttagttgctacccttgagaaggcccctgactaccttcattaaagacacatgtaagatgggtaa
gcaagtcaataagaactatgcattgtctctaaccagttgttcatccttgatgcactgttcaaatgtgccacaagcttccttagcttcagataccttgccc
tttatattgctttctgtgtcaaagctcatcccaagcacttgtaagaaactgtccagtgctgtaagtcacccctggtcgatatgcctgtctggaaggtagt
gatgttatccttgccatttgtaactactgg
3' EST: CX976283 [+]ttttttaaatttttttggtattttacttccttttatgaatttactcattttttttgcctatgtctcacaacattactgagaaaagacagatattcatccg
cattttacattcatttcacagatgtgagcaaggattccaggtacccagcattcatgatgggaaaacataaaaaagaaaggaaagagaaaaataaattgtt
caccaaaccagaggtgggtcgatagtgaaagaacccaaaccgtttgcagaacacaccaaggatataaactgtcatgagttcggcaagggttccagttggg
tgaacagaagtgccatagattgaacacgatatgagcaactataaggggaaaaagtggggctttgcgtttacaccaagagtcatggctgaacttaatctcc
tcagcagtttcagattcctgggaagggggacctagaaattatacagcagcactaaccttagtctttggatatcagatgctgaattcccagccatgtattt
ctacccagtagttacaaatggcaagataacatcactaccttccagacaggcatatcgaccaggggtgacttacagcactggacagtttcttacaagtgct
tgggatgagctttgacacagaaagcaatataaagggcaaggtatctgaagctaaggaagcttgtggcacatttgaacagtgcatcaaggatgaacaactg
gttagagacaatgcatagttcttattgacttgcttacccatcttacatgtgtctttaatgaaggtagtcaggggccttctccagggtagcaactaaggat
tccctacaatttggcaccctgaactgagcattttctttcagtttccgtaagtaccactcaaggggggcaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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