XB-CLONE-3239997
Clone Name: IMAGE:7716133
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Gene:
eps8l3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976380 [+]
gaacagccggctgatgttcttcatgcaaacctagaaaaggcattaaagcaattcagattaaacaaagaaagcaaggatacctacaggtataacttggaga
actctcaccctcaaaataattttttagaaaggagtcaatcaccacaatccagtggaggctgggacagtcccagaatggaaaggcaaccaaaatactcccc
ccgtgaatcccctattcagcagaggagatttgagctctctgaaccagtccatcctcctccaagcaccaagcctcctccatccttccaagcagcccctgtc
aattcaaagtcagagacataacgtgacattgaaattttgaatcatcttcttggagacattgagttatttgttggaaaactgaatattgataagaagaaaa
agaaagcaaagcttcctgaatcagaatttgtagattgtctgcagaaagctaaatatgcctttattctaatggggaatgtccacgaacaaatgaaacaacc
aagtgctccagatctcatccatttgatgatggaaaccctggccaagattgtagacaggtgtccacaaaaagacttgccacagtctgtgttatctccactg
cttacacagaaggcactgatgattatgagttcttgtgtgacagacaaagaaagaaaattctgggaaagcctgggagatgcctggatgaagaccagagctg
aatggccaaatggaaagaatatacctcaatatgttccaactttttctgatgggtggatcatccctgatatcccctttagtgatgtttcatcccagatgtc
acgtagtcctgaaacaccactacgaaacaggcaatttcacccactcctcatgaatgtccttca
3' EST: CX976379 [+]cgagaggcaaaagttcaaactttaattctgaagtagagaaactgatctgcctttttaaaaggtataattttatttgaactgatgtgttgcaaatactggt
tccaaaaaaataggaaaaattcaaactaaaattaatcttggaatcctgttatattatattgagttatattgaggttttctttttaatttctgaatggttt
gacttgttattcttcagttttcattctggaaggcttgcaagggtcagaaaccaaataattcccccagtccttttcttcatagtacctgaccatcatctgt
gttctccaggatgttgcttggaataaatccctgctggccctgagaattctgcaccatccaccactgccggctctggtccaaaaccttcacaatgtctcct
ttccttactgacagttcgcgattatttctcgcttcaaagtcatgaaggacattcatgaggagtgggtgaaattgcctgtttcgtagtggtgtttcaggac
tacgtgacatctgggatgaaacatcactaaaggggatatcagggatgatccacccatcagaaaaagttggaacatattgaggtatattctttccatttgg
ccattcagctctggtcttcatccaggcatctcccaggctttcccagaattttctttctttgtctgtcacacaagaactcataatcatcagtgccttctgt
gtaagcagtggagataacacagactgtggcaagtctttttgtggacacctgtctacaatcttggccagggtttccatcatcaaatggatgagatctggag
cacttggttgtttcatttgttcgtggacattccccattagaataaaggcatatttagctttctgcagacaatctacaaattctgaatcangaagctttgc
tttcttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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