XB-CLONE-3239979
Clone Name: IMAGE:7716107
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Gene:
aasdhppt
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161572 [+]
ggaagtacagctgaggcagaatagccacagtgctcggcagggggtcgtacgtgccgtagctgaggaactttccttcccagttgtagaaatacacatgcag
ttcagtgccgtaaagcgctgcgctatggggagcgtacggtgggcctttcactgcagctcttgggctcccagccaggctgagtggcttctgtgtgcgcggt
gcgttcagccagaggagaaggagcgcattgggcagttcatgttcactcgggatgccaaagctgccatggctgggcggcttttgatgagaaaagtaattgc
agaaaagctccagataccctgggacaaaatattgttgcaaagaacagaaaaaggaaagccatttctctgttatggaagttctagcgagtatcctttattt
aaatttaacgtgtcccaccaaggagactatgctgttcttgctgctgagccataccgacaggttggagtagacattatgaaaactgatttgccaggcagtg
gctcaattaaagaatttttccggcttatggatcggcaattcacagaaaaagagtggcactcgatcagaagtatgaacagtgattgggctcaattggacat
gttctacaggcactgggctctcaaggaaagcttcataaaagccattggggttggactaggcttcaacctgcaactgattgagtttgaagtgtcgcctgtg
aatatggatataggcaaaacttacaaacagaccaagatgtttttggacgatgaagaggaaacatgggcatttgaggaaattctgctggataaccaacatc
atgttgccgttgctttgggagaagtggatcaacttctgcatcagagtcacaagattaatgacgtgacagagagcacctttgaacttctcagttttgaaga
cttgatggcatctgctgtcccaatgtcagacgaagatccagattactggattaactttcagtcaaaacaggaatttccttttcgccaaagaaccaatgta
aaaaaagagacttctgtatagtaaaaatatgtatacttagagaaactgtcaatattataaaactaaacttcagtagaagtttaagcggatggtcaccttt
ggacaaaagtctgcatttgaacaggccctgttagaaaaagcatttttgtaaaacatctttattatttaaaatgtgtagtgttgaagatcaggatcagcgc
tgctcactgatctctccctgctctcagtaggggatttctcctatgagtacttggcaggtagccaaaaaaaaaaaaaagttgacattgaactagttactgg
ccataacttccctctcctgcttcatttgcatgtctttgataccattggctggcatgattcagccaatcggatcagagaggtgcaatgaaacagaagaggg
agggcaatcactttagtatcaacttcctgttttgggcagaatgccagctgaagtcagagatcccttcagaagacagtggagagagtctgtcagcagcagt
tatcctggtctttagcttcaaaactacacatttgacaaacatgttttttcttcagattcagactatttttaaaaaaatgtttcatgtgcaacgtttatat
tgttcagttatattttttccggaagttgtatatatatatatatatatagatatatatatttataaatattataaattattttttgaccaatttttttttg
cttgtttcaatctgtagatgacatctttattatttttatataatttaatttatagtatctttgttattgttccttggtatgtctttgcccatagtccttt
ttctttgcaatcatccctcttcactatactacacccaaccaaatcagtgtgtgtggactttccctgtgtccccatctctgcctatctagatgtcttcact
tctcttattaatctaccctgtggcataatctgtggcatcagcagcagccatcattcatgggatgtgtaccacttctgcactgggtggcagaagtagactt
tgtgtgctgctcagttctgcctctgaggatctgtgaactaacaaggtatgaaataaaatcagagaccatagatcagaggatttcattggtctacatggag
atttgaacactttgccagggacctttactgtcttaaataaaactagttatttacaaaatgcttacaggaatggcttgcagtctttttaaaaaaataccaa
cccccagtcaccattccggggccaatactgtatatttggtgttagaatgggaactctatctctgcaacttcatgtataacttaatgcaagaagaaggcaa
accatttatacagcttcttggttttaccaaggaagataagttttttttttcctttgatttgtcacatacatggatatgtggctagataaaagcctgaatt
agttaaataaacccaagcccatgactgatggactgtcatactggaaagtcattccaccaaggaaggtttacaggtgttggcttagacattcgggagaccc
tagtttgccttgactttgcaagtcattttgacctgggggaaatttcttgttggctgttgtggcatggttgcaaatttgggaaagatcactttgccaaggt
aaaatttcacactatagtgatttatgttcaaacttctgaattcttttcacttctatgcaaatattcttcatagagaaaattatcttttgaatgtactgca
ggcctaaatgtttaataaagttttgttcaattgggaataaaaaacaaaaaaaaaaaaaaaaaa
5' EST: CX976530 [+]gaagtcagctgaggcagaatagccacagtgctcggcagggggtcgtacgtgccgtagctgaggaactttccttcccagttgtagaaatacacatgcagtt
cagtgccgtaaagcgctgcgctatggggagcgtacggtgggcctttcactgcagctcttgggctcccagccaggctgagtggcttctgtgtgcgcggtgc
gttcagccagaggagaaggagcgcattgggcagttcatgttcactcgggatgccaaagctgccatggctgggcggcttttgatgagaaaagtaattgcag
aaaagctccagataccctgggacaaaatattgttgcaaagaacagaaaaaggaaagccatttctctgttatggaagttctagcgagtatcctttatttaa
atttaacgtgtcccaccaaggagactatgctgttcttgctgctgagccataccgacaggttggagtagacattatgaaaactgatttgccaggcagtggc
tcaattaaagaatttttccggcttatggatcggcaattcacagaaaaagagtggcactcgatcagaagtatgaacagtgattgggctcaattggacatgt
tctacaggcactgggctctcaaggaaagcttcataaaagccattggggttggactaggcttcaacctgcaactgattgagtttgaagtgtcgcctgtgaa
tatggatataggcaaaacttacaaacagaccaagatgtttttggacgatg
3' EST: CX976529 [+]attcccaattgaacaaaactttattaaacatttaggcctgcagtacattcaaaagataattttctctatgaagaatatttgcatagaagtgaaaagaatt
cagaagtttgaacataaatcactatagtgtgaaattttaccttggcaaagtgatctttcccaaatttgcaaccatgccacaacagccaacaagaaatttc
ccccaggtcaaaatgacttgcaaagtcaaggcaaactagggtctcccgaatgtctaagccaacacctgtaaaccttccttggtggaatgactttccagta
tgacagtccatcagtcatgggcttgggtttatttaactaattcaggcttttatctagccacatatccatgtatgtgacaaatcaaaggaaaaaaaaaact
tatcttccttggtaaaaccaagaagctgtataaatggtttgccttcttcttgcattaagttatacatgaagttgcagagatagagttcccattctaacac
caaatatacagtattggccccggaatggtgactgggggttggtatttttttaaaaagactgcaagccattcctgtaagcattttgtaaataactagtttt
atttaagacagtaaaggtccctggcaaagtgttcaaatctccatgtagaccaatgaaatcctctgatctatggtctctgattttatttcataccttgtta
gttcacagatcctcagaggcagaactgagcagcacacaaagtctacttctgccacccagtgcagaagtggtacacatcccatgaatgatggctgctgctg
atgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: