XB-CLONE-3239940
Clone Name: IMAGE:7716150
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Gene:
eef1g
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976834 [+]
ggggttccccatcaaggttgcctcctctcctccagaattccagtttggagtaacgaataagacatcagagttcctaaagaaattccccttaggcaaggta
ccagcatttgagggcaacgacggtttttgcctttttgagagcagcgccattgctcattatgtgggcaatgatgagctccgaggaaccaatcgtttacacc
aagctcaggtcatccagtggctcagcttctcagacagtcacattgtccctccggccagcgcatgggtctttcccactttagggatcatgcaatacaataa
gcaggccacagaacaagccaaggaggagataaaggctgtgctgggtgttttagattctcatctgaagacacggacattcctagtgggcgagagggtcacg
ctggctgatatagcagttacttgctctcttctgtggctctataagcaggtcctggaaccatccttccgccagccatttggtaatgttacaaggtggtttg
tgacctgtgtgaatcagccagaattccgtgctgtgttgggagaagtcaagctttgtgacaagatggcacagtttgatgctaagaagtttgccgagatgca
gcccaagaaagaaactcccaagaaagagaagccagcaaaggagccaaaaaaagaaaaggaggaaaagaagaaggcaacaccagctcctgctcctgcccca
gcccctgaggatgatatggatgaatctgaaaaggctttagcagcagagcctaaatcanaggacccatacgcacatctacctaaaagctcttttgtaatgg
atgagtttaagagaaaatactctaatgaggataccctggctgtagctctgccttatttctgggagcactttgac
3' EST: CX976833 [+]tgctgtcttgttaagttaaaggctctggatgttggtacggatgtgaaaaccagactctgcgcgtattttagggggacagagatcacctcctcctcatttg
aagatcttgccctggttaaaggcctttcccacgtgcttgaagtctccctcccagcagaagtattctttgaccagggttttgcactcctcactattgctgt
ccagctttctccaggtgtaggattcatagtcaatttgccagtcttcagagagtgtgaatgcaaggtcctggcccctgaacacccacacgccggagatgga
gctgtcattgttggtgccaaacaagatgacgctggcaaagccagtcttgcgcagtttgtctagacgctggaacatacctgtaattaagttgcagctcatg
aaggtttgtgtcagctcctcggggaatttgtattcagcgtaccagatggaccagccttccttgtcaaagtgctcccagaaataaggcagagctacagcca
gggtatcctcattagagtattttctcttaaactcatccattacaaaagagcttttaggtagatgtgcgtatgggtcctttgatttaggctctgctgctaa
agccttttcagattcatccatatcatcctcaggggctggggcaggagcaggagctggtgttgccttcttcttttcctccttttctttttttggctccttt
gctggcttctctttcttgggagtttctttcttgggctgcatctcggcaaacttcttagcatcaaactgtgccatcttgtcacaaagcttgacttctccca
acacagcacggaattctggctgattcacacaggtcacaaaccaccttgtaacattaccaaatggct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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