XB-CLONE-3239930
Clone Name: IMAGE:7716162
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Gene:
pola2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976918 [+]
gaacatatgcatggcagtgctaagaatgcaaatcgatgttttgggcctgtgcagtgtatatatctcaaaagtggcttctcaattggatatccaaaagttc
atctcttgcagaaatggatgatgggaaattgcatttcttagaaagttccaaagaattgactttacaggagaccaggtttaagtgaattagtttaaagaaa
cacaggtaaaaatatacaaatccacaaaatgatgatacagccgtctgcaggctcctttcagagcactcgttaattacatgggctctttatgtacattgtc
ttcaccccagttctatttgtcagatgttaattcagttctcaggtttccccagttagatcctcaccacctgagcagaaatacaaggagatctctttccttc
agaccccatggctccacttttgaccagaaagcgtgcatatgtgccgcccaccagtccctttgtcagccgcccagggttaatgcagatgcagcctgtgaca
tccttgataaaatagcgaagctcagacgggactataaaaatgtctggagtgacaggcataggggcgtagctgtagaaagcctcatagtcaacatttattt
cttcattgggtggatacagtggatagtaacttctttgggtcagaatgtgtctaagaatccgactaaatcgatccggagcccccacagagctgcttatttc
ctctgcacccatatgaaagagcaagtctgtagaggttatgcctaagataacaccattgacagacaacgtgcagggatcggcaacaaaatggaccctgtcc
ttgtcttctttggccggttcaaaacagctgaatggtggctgaggatagacaggatcatgatgaacatctctgagagatggaac
3' EST: CX976917 [+]ggaaatttcattcggatagaaccgagattccgcgatttgcaagtaccagcctgcctcccgcatcaaggatctgccatcctttgagcctacaactggtgaa
gccatgcctgtgtcagcaaagagcattgaagaggagctaacggtctttgacgtcaactttgaggatgatgaagttccagagaaaatggtggagctctgta
cagttcaccgtcttcaggaagaggacatggtcaatgaatggatggctttcagtaccactcggaaccttccgctcacagtcagcaaccttaatatactgga
acatgaggttttgaacaagaaaagtgccagaccacgcccatcccagaagaaagagaaacattgtggaaacagggattttaatacaattcaagaacttatt
gaagtggaaacagaagaagaaaatctcctggattcatatgctacacctgctaaaggatcccaaaagcgtaacttgtctactccagaacatcctcagtcta
aacgcatatctgtaaaccgaagcccccatgtcctcttctccccagcaagcttttcccccagtgccaccccgtctcaaaagtatggctctcgaaccaaccg
aggagaagtggtgacaacctatggagagttacaaggaactacatggaatggtggcagtggaagaaatactagtgttgaactgttcacctgtcaggatgaa
ccactgacaaaaatgtataagttcatgtttcagaagttaatggacatcagagaagtggttagcagtaagatagaagaacttggtgcatctctgaagaacc
atttccaaattgatga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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