XB-CLONE-3239906
Clone Name: IMAGE:7717085
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Gene:
cbx4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX977698 [+]
gcgggacatggatatatccagacctctcttatacagcctcctgctgctcacagtaacaattctccatttaatttttacatccaagcagctacaggaagca
ttaacaattctaatagcagcatatactagaaagagctctcatttttttttgcaaacaaaatataaataaatgtttaaaaaataaaaaaaaaaacgtatat
aaggttacaaagtctgtcctctctctctttcacgtgtataagaaggcggaacactgaacagctaacacgattctcccagacagaacaaggacaccgaaga
gccgatcatttccaggtataaatataaaataaataaacacaattaacctagttttcaatcaaaacagacatggatttaagtgctttgtcatagtgatcct
tggatggcacataagttattagaatccagccactgctaggcccttccagcaactctatatatatataatatatccatagggaagattaaaaatcatgccg
actggctggaaagtgtcagtttagtatatgcagttcagaaggaaacacggtttccctgcatatagttttaagaatcacaccgttatatattccttaaaag
tgaccgtaaggcaattggctgtgacatctgtaattactatatttccaaagaaaggtttaaattcagactcacaaacatccacatcaactgactgaggttt
ctgagtgacttggacctcaggtttttccacctcttggtcactgtcacaccgagacttcacacagcgcaggtctatgggttcatccaagtcggagtccaag
agaataatatct
3' EST: CX977697 [+]cggacctggactggctgctaaggcgcaacagtggcggaaagccaggacataaggaacatactggatattagagtttgacccttgcttgtgtgagagtggt
agaacagacgcaatctgtccggcactgtaggggctttgggggcgcagcagtgcggagcagcgtgcagttttgcgggtgtcctggactgggcagttccgca
aggaccggggagcaccgagcgggattgacagcggcttgttcttccagcgggactgagctccagcccctcactgctggggaacatggagctgcccgccgtc
ggagagcatgtgttcgcggtagaaagcatcgagaagaagcggatccgcaagggccgagtggaataccttgtcaagtggagaggatggtcttccaaatata
acacctgggagccagaagagaatattttggatccaaggctactggttgcctttcagaacagggaaagacaagagcagatcatgggatataggaaacgtgg
ccccaaaccaaagcatcatattgtttctataccgtcctttgctcgccgctccaatgttttgaatggacgcaatgattcgcctggtgaaaaccgaaccaag
ttggacctagggcctatccccaagagccagccccatcaatatcagctaaacagtaaaaaacatcaccagtaccagcccagtggcaaggaccacaatgaan
agcatcattcgaataccaagaagaaatactattatcagctcaatagcaaaaaacatcatcactaccaacctgatcctaagatgtatgaacagtatgcctt
gggaaaagagtctcanatcagtactgatcccagtaacagacacagagagtcctgggcacacacaaaggctgcagatgtagggactcaaatga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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