XB-CLONE-3239846
Clone Name: IMAGE:7717329
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Gene:
actg1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX978402 [+]
ttggaagttacaattctcatctggatgacatacaaattttggttttctacgttgtgagaacattaaggcaacatacaaaaaaatttacataaattaactt
ggatgcataaatttacaggtgttaactgcaacatttgtaactttcttcagacccattttgaatgttgggaatacaacctgggttaagaaaaagactggtt
cttgggcctgagtgtcaatttccaaccttactataaactggaaagatagaagagattccttcatgaactgaccagcctccgacatgaaacaatggagtat
taagcgcttaactgtgtgtggaagtctaaacctcagataccctgtacacatctccaaaactcgacttctccataaacagcccctgtaacctagaggagaa
gtgtcattcgcaatactgtcgcagatcggcagcctttcacagaaatgcttctttctacgtaaagcttagcttaagtgtcacccagactgtatgccaacca
ggcaatataagagatgtgtacagggtacaatatgctagcattgatgccacaggaatgatttgaagagacaagcacaagtcttgcattacaaaatccccca
acagtgactatgtgtgtctccaattcctaggataagttcatttggaagttggccctttttccagtttcaagaggctagcatgaggcgtacacatttgcca
aggcaaattaatactttgaagaactcacgcagcanatgcaacgcatctgctagcagtccatttagaagcatttgcggtggacaatggatgggcc
3' EST: CX978401 [+]cgagaggctttgcatttctcctcttaaggaaacctccagcaccaagaaataaaatggatgaggaaattgcagcactcgtcgttgacaatggctctggaat
gtgcaaagccggctttgctggagatgatgctccccgtgctgtgttcccctctattgttggacgcccaagacaccagggtgtaatggttggtatgggccag
aaggacagctatgtaggtgatgaagcccaaagcaagaggggtatccttacccttaagtatccaattgaacacggaattgttacaaactgggatgatatgg
agaagatttggcatcacaccttctacaatgagcttcgtgtagcccctgaagagcacccagtcctgcttacagaagctcccctgaaccccaaagccaacag
agaaaagatgacacagatcatgtttgagactttcaacaccccagctatgtatgtcgccattcaagctgtgctgtctctgtatgcctctggtcgtaccact
ggtatcgtgatggactctggagatggtgtcacccacacagtacccatttatgaaggttatgccttgccccatgctatcctgcgtctggacttggctggtc
gtgacctgacagactacctcatgaaaatcctgaccgaaagaggctacagcttcaccaccacagctgagagagaaattgtgcgtgacatcaaggagaaact
ctgctatgttgctcttgactttgaacaagagatggcaactgctgcatcctcctcatccttggaaaagagctacgaactgcctgatggacaagtaattaca
tttggaaatgagaggtttagatgcccagaagcactcttccagccttctttcctgggtatggaatcatgtggcatccatgaaactacattcaactcaatca
tgaagtgtgatgttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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