XB-CLONE-3239596
Clone Name: IMAGE:7719978
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Gene:
gpt
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983715 [+]
gcgacttccaaattgcacatacactttgctcatcattactccaagtacaggagaaatcagcagggcaggaagattagaccataggcacctgttccattct
tccaggagcttcttgcaggagggcaacaaacctcttggactaactctctgttaattctaatgctgtgggtgcattcaaaggacaggcgatggttgcttga
aagcactggataatgcatttattagctttcgcagccccctcgactggatacaaagacaggttaagatggcaccgctgcacagttctgcagatttagggct
ggatcgcacacgggaactttttgaactgaatagatgttgcttggagggggctcaatggagaaggagttaaggatgagctagggtttagttctcctttagg
tgctcatctctgagatatgtgggtgacttgctccaatgtggatgctgcactctccactgcatatattgctatggtgagctggtgatggaccatgtcccaa
tcgtcctgtgagttgctagcggtggcttgaaggtaggcatcagcaaggcctgggaaagtggctacgccggaactgcgcggtgcccagatgatgtggctgt
aatataactgctcagggaaagccatgtggttaatgaaagttctttccagcagcattaactggtcattaatcatgcggattttaagtggtgtctccttcgt
ttctttcagctcttttatggtttcctccaaagcctcgcatgccttcttgtatctatttattgccattttcaggggtgccagagagatgtttttgctgacc
aggttggactgaaagctttgctctgccctgtt
3' EST: CX983714 [+]gctaaacaggactggccagtggtttggctgcttggcgccttggctagaaggtccgttagggaaccggcggatctggcgacagtgagagatgactgagcag
cagccaaggacgaccagacagccaagaatacttaagactttggtgctaaattgccctagtgcagttgcccatagcaaccaatctgaataataaagtggag
tgattggaatgggcaagtgcatgggtgcaaccagaacctgttactcagcgggtccttgggtgtttctgatgggttgataaaaatgcaaagctcatcctga
actttattttgcatccaacatggccgcctccggagaaagcacaagtgtaggcattccagtttgtcatcagagacattgcatagcaccttccatgcattcg
tattatcgctaaattccgagcagcgttgggaactttgaggtgccctcctaggtacaatatcaggcaagcccaaacttagaaaggacacttagggacttca
gcagggcatcggagctggattaaggcctacggagctcagccacccaagggcctgaacgtaaagcttccatgatgctacattaatgggttaattatgtcta
aaaagcaagagattaagcaacgagggccataaggggacccaataaggcctactgtactgcacccacattggtcaataaaacaccctaaaagcagagttac
ctctctacagctactagccagcagggccctagagaagagacagatggggcaataggctgggaaataaganagtgcattgagtggtcattgngcggccccc
ttagcgaggcacagtcattcgtgtaaggaaacgcggagctcgttcaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: