XB-CLONE-3239588
Clone Name: IMAGE:7719906
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Gene:
ephx1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983892 [+]
gaatcgcacgagggaagaattgaaggatttatttgatcgtattgaccatacccgatactctgagccgctggaaggcagccgctttaattatggctttaac
tccacccatctcaggaaggtggttgcctactggagaaatacctttaactggaggaaacagctggacatcctcaacaagttcccacatttcaaaaccaaaa
ttgaagggcttgacatccactttattcacgtgaagccaccacacctgcccgggggtcgcaccgccatccccattataatggttcacgggtggccgggatc
tttctacgagttctacaagatcatcccgttcctgacggatccagggagccacgggttggacactcagtacatcttcgaggtcatctgcccttccattccc
ggctacagcttctcggaggcctcccacaaacaaggatgcgaatctgtggcagttgcacggatcttttacaaactaatgcgacggctcggcttccgggagt
tctacgcccagggaggagactggggatccttgatctgcacaaaccttgcccagatcgctccaaaccaagtgaagggactgcacatcaacatggctgccgc
tttccccaccaaactgccgcagattctgtcagttgtgctgggccagcgcttcccggggctgtttgggctgcatgacgaggatgttcggcggatcttccct
ttctttaagaaggtggtgtcccatgtaataaaggagagcgggtacatgcacctccaggccaccaagcctgacaccgcaggctgcgggctaaataactccc
cagttggactcgctgcctacatcct
3' EST: CX983891 [+]gagacaagcctgtaatatattattattataactgtatcattttgtatgcaatgtatataaatatattttgcaaacctgattaaagtgtaaaatacaccaa
gtaagagtgagaagtcatttatatttgcgcagggaccagcccaatgttcagcagcagcctcgggctctcgagcacagctgaggattctgggagttgcagt
tctgcagatggagtttgaactgtcatgtttggctgtgccaaaacctttgccaacaacctccttgtaaaatctcattgaggaaacaatggagccgctcagc
cagtagatcatgatattggtcagtaggtcgtccaatgagaacttcctctcaagtcctccatcctcatgatcgcggaaatccgggtcggtccatgtagaga
acttctccaggatgtaggcagcgagtccaactggggagttatttaggccgcagcctgcggtgtcaggcttggtggcctggaggtgcatgtacccgctctc
ctttattacatgggacaccaccttcttaaagaaagggaagatccgccgaacatcctcgtcatgcagcccaaacagccccgggaagcgctggcccagcaca
actgacagaatctgcggcagtttggtggggaaagcggcagccatgttgatgtgcagtcccttcacttggtttggagcgatctgggcaaggtttgtgcaga
tcaaggatccccagtctcctccctgggcgtagaactcccggaagccgagccgtcgcattagtttgtaaaagatccgtgcaactgccacagattcgcatcc
ttgtttgtgggaggcctccgagaagctgta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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