XB-CLONE-3239532
Clone Name: IMAGE:7720734
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Gene:
rpl9
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995609 [+]
ttcttgcaatttctgtgattctggtcacaatctgcgtattgacagaatgtaagccttcagaagcagactcggaatctaagagcttcagggaccactttga
ttctgcagggactgcaataaaagattttggagccaaaaccaaggattttttcaaggatctgcataacagtgaggctatgacaaagacaaggtacgttcat
gacctcctccagttcagtggtattttctaaacagacagtggagactacttatgtttttggttccgttgatggttagggtgatggaagttggaggaagtca
gattgttggaggcagtagcggtagatgttggaacttggatagcaggcagtagaggaatgtggtgggagctggatggcaggcagtagaggaatgtggtggg
agttggatagcaggcagtagaggaatgtggtaggagctggatagcaggcagtagagcaatgtggtgggagtcggatagcaggcagtagaggtaatgtggt
gggagttggatggcaggcagtagaagtaatgtggtgggagttggatggcaggcagtagatgtaatgtggtgggagttggatggcaggcaggcagtagagg
taatgtggtgggagttggatggcaggcagtagaggtaatgtggtgggagttggatggcaggcagtagaggtaatgtggtgggagtcggatagcaggcagt
agaggtaatgtggtgggagtcagatagcaggcagtagaggtaatgtggtgggagttagatagcaggcagtagaggtaatgtggtgggagttggatggcag
gcagtagaggtaaagtgggtgaaagcccagttgcaggcagtagaatgaaaagctatgtaatatggggtcccaggccct
3' EST: CX995608 [+]gtgacaaacgtcaggtttatttattaatggagatcagtgggggttactccattaggctaacacaactggaacacattgaggcacattggtggtcagaaca
tgagggtctcttggggaagaatcctgctggtcgggtctaggatgcattagtctgagccggttgttttctcctttatcttcttgtaaaggtctgtgaacca
gttcctggcagaaataaagaaaggagtattaaacctgcacatgtgccgggagagcttggacaaagtgtgagggctggtacatgaaccaggacaatattta
ttaacagatctgatattggtctgtaggtcctaccctgcctcctttatgtggacctgtagttccatataaaagcctttacctgtacttcttgcaacacaac
ttcacctgaatttaccttagggcctggaccccatattacattagctttcattctactgcctgcaactgggctttcacccactttacctctactgcctgcc
atccaactcccaccacattacctctactgcctgctatctaactcccaccacattacctctactgcctgctatctgactcccaccacattacctctactgc
ctgctatccgactcccaccacattacctctactgcctgccatccaactcccaccacattacctctactgcctgccatccaactcccaccacattacctct
actgcctgcctgccatccaactcccaccacattacatctactgcctgccatccaactcccaccacattacttctactgcctgccatccaactcccaccac
attacctctactggctgctat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: