XB-CLONE-3239452
Clone Name: IMAGE:7721002
|
Gene:
slc7a7
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX996502
[+]
ctcatattccaacatgggtacctctaccaagtacacacctgcctccacagaagagccaaaccgtaatgggacaaaagattccacagcagactcgggctcg
caagagtccatgaaactgaagaaagagatctccctgttaaatggggtgtgcctcattgtggggaacatgattggctctggaatctttgtgtcacccaaag
gggtcttaatatacagtgcttcatttggcctctcactggtattatgggcagttggtggtattttctctatctttggcgctttgtgttatgctgagcttgg
gactacaatcaccaaatctggggccagctatgcctatatcctagaggccttcgggggttttgtggctttcatcagattatggacatctcttctcatcatt
gaacccacaagccaagctgtcattgccatcacattttccaactatatgttgcagcctatcttcccttcttgtggatctccttatgctgctaatagactca
ttgcagcaggttgtatagcactccttacctttgtgaattgtgcctatgtaaaatgggggaccagggtccaggatatcttcacttatgccaaagtgctagc
tctgattgctgtcattatagctggaattgtaaagatctgtcaaggtcattcagagaactttgaaaattcgtttgctggctcctcatacgatgttggtgac
attgctcttgcattatattctgcacttttttcatactcaggatgggacacacttaactttgtaacagaggaaataaaaaacccagagaggaacctgccat
tggccatt
3' EST: CX996501
[+]gaataactgttatcaacacaactgctttattttagatggattaactgtttcatccaggagcacaaaaacttcaaaaggttttttccttccactggcatag
ccaaattccacaataaataactttgatttgaagggaaataagaaatcaatagtgtagatggctcccctttcgtgttggattagccaggctcatattgctt
tacaacacaaggctggcccttagatcagaaagtagttaattccttgctttggaaataataaaaggtttaatatttatgctattcaactattccctcccag
agagaaatggtttgcccatgtccatccagagaactgctctgcagcaaccttgcaatagaaaggtagtttttttttcttttttaaaaaaaggcacagttta
tgcaataaattagtaaggcttcaggtttctttgagtcagccatggaatggtggacagttctatcaatcactttttgagaagtctttttcctcttctgtat
ccatttcagttagtactgagtagcacagaatttgtgtatatcgtgtcagagcacctgcagcacgctgaagacattgtggcttccgtttctcagaaacacg
aaccagaaggaaatagactgggactcctgacaatgcaattcccactccaatcagagaatttattagatcactatacaggggtacagccaccaggaacaca
gtgcaaagacagaatatgattgggaacactaggctgagcttgagtggccttggtcggtctggttgtttccagcgcagatacagctgtcccgcaatagaaa
gtcccacaaataaccagtaactgaaactgtagtaattaatgagctggaaatatcttncacacagaggta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: