XB-CLONE-3239443
Clone Name: IMAGE:7721248
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Gene:
u2af2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122000 [+]
CATGTCTGACTTCGACGAGTTTGAGCGGCAGCTGAATGAAAATAAGCAAGAAAGGGACAAGGAAAATCGTCATCGAAAACGTAGTCACAGCCGTTCTAGA
AGCCGAGACCGGAAGCGCCGCAGCCGTAGCAGAGACAGGCGAGGTAGAGAGCAGCGAAGTGGATCCAGGGATCGAAGGCGACGCAGTCGCTCACCAAGAC
ATGAGAAGAAAAAGAAGATCCGTAAATACTGGGATGTTCCACCTCCTGGTTTTGAGCATATCACCCCTATGCAGTATAAAGCCATGCAAGCTGCGGGTCA
GATTCCAGCTACTGCTCTCCTTCCAACAATGACACCAGATGGCTTGGCAGTTACTCCTACTCCTGTGCCTGTTGTGGGTAGTCAGATGACAAGACAGGCC
CGTCGACTCTATGTTGGAAATATTCCATTTGGAATCACTGAGGAAGCAATGATGGATTTCTTTAATGCCCAGATGCGCCTTGGTGGATTGACTCAAGCGC
CTGGAAATCCTGTACTGGCTGTTCAGATCAATCAAGACAAAAACTTTGCCTTCTTGGAGTTTCGTTCTGTTGATGAAACAACACAGGCAATGGCTTTTGA
TGGGATAATTTTTCAAGGCCAGTCTCTCAAAATAAGGCGTCCTCATGATTACCAGCCTTTACCAGGCATGTCTGAAAACCCATCCGTGTATGTACCAGGG
GTGGTTTCTACAGTTGTCCCTGACTCTGCACACAAGCTTTTCATTGGAGGTTTACCCAACTATCTGAATGATGACCAGGTAACCATGGAGTCATTAAGCC
TATGGGTCAAAGAATTGCTTACATCTTTTGGGCCTTTAAAAGCATTTAATTTGGTAAAAGACAGTGCCACAGGCCTATCAAAAGGCTATGCCTTTTGTGA
ATATGTGGATATTAATGTCACAGATCAGGCAATTGCTGGACTGAATGGGATGCAACTGGGTGATAAAAAGCTTTTGGTACAGAGGGCAAGTGTTGGAGCT
AAGAATGCAACTCTGAGCACAATAAACCAGACTCCCGTGACCCTTCAAGTCCCTGGTCTTATGAGTTCTCAGGTGCAGATGGGTGGTCATCCAACAGAGG
TGTTGTGCCTAATGAATATGGTGCTGCCAGAAGAGTTGTTAGATGATGATGAGTATGAGGAGATAGTGGAGGATGTTAGGGATGAGTGTGGCAAATATGG
CGCTGTCAAATCAATTGAAATACCACGTCCAGTGGATGGAGTTGAAGTGCCAGGATGCGGCAAGATCTTTGTAGAATTTACGTCAGTCTTTGATTGCCAG
AAAGCCATGCAAGGACTCACTGGGCGCAAATTTGCTAACAGAGTGGTGGTTACAAAATACTGTGATCCTGATGGTTACCACCGCCGTGATTTCTGGTAGA
ATGGAAGCGAAATGGCGGGCTGAGATGAGCTAGGATCGAGAGGCTGGGGGAATATCAGAAGGACCTTCATTTTATTTTATTTTTTTCTTTTGCAGGAGGC
TTTTTTTTTTTCTGATTGTGTTTATATTTTTATGGCCAAAAGTAATTTTTTCCCTCCTTGAAACATCATGACCACCTCACTAATCCTTTTAAGGAATTAA
ATTTGAGACCTATTCTTTGACCATCACACCCCCCACACAATGTAAAATAACCCCAGAACCTCCCCTTCCCCACTCCCCCTTCTTGCCCCCAAGGACCATA
AAGAATCTCAGTGGTGGTTATACTGTAGTAAAATGTTGTCTTTTCTTGATTTTCTTTCTGTGACCGTTTCATATTTGCAAAGACAAAATTGCTCATTAAA
CATTTTGTTGTATTTTACTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX996558 [+]catgtctgacttcgacgagtttgagcggcagctgaatgaaaataagcaagaaagggacaaggaaaatcgtcatcgaaaacgtagtcacagccgttctaga
agccgagaccggaagcgccgcagccgtagcagagacaggcgaggtagagagcagcgaagtggatccagggatcgaaggcgacgcagtcgctcaccaagac
atgagaagaaaaagaagatccgtaaatactgggatgttccacctcctggttttgagcatatcacccctatgcagtataaagccatgcaagctgcgggtca
gattccagctactgctctccttccaacaatgacaccagatggcttggcagttactcctactcctgtgcctgttgtgggtagtcagatgacaagacaggcc
cgtcgactctatgttggaaatattccatttggaatcactgaggaagcaatgatggatttctttaatgcccagatgcgccttggtggattgactcaagcgc
ctggaaatcctgtactggctgttcagatcaatcaagacaaaaactttgccttcttggagtttcgttctgttgatgaaacaacacaggcaatggcttttga
tgggataatttttcaaggccagtctctcaaaataaggcgtcctcatgattaccagcctttaccaggcatgtctganaacccatccgtgtatgtaccaggg
gtggtttctacagttgtccctgactctgcacacaagcttttcattggaggtttacccaactatctgaatgatgaccaggtaaccatggagtcattaagcc
tatgggtcaaagaattgcttacatcttttgggc
3' EST: CX996557 [+]aaaagtaaaatacaacaaaatgtttaatgggcaattttgtctttgcaaatatgaaacggtcccagaaagaaaatcaagaaaagacaactttttactaccg
tataaccccccctgagattctttatggcccttgggggcaaaaagggggaggggggaaggggaggttctggggttattttacattggggggggggggtgat
ggtcaaaaaaaaggtctcaaatttaattccttaaaaggattagggggggggtcatgatgtttcaaggagggaaaaaattacttttggccataaaaatata
aacccaatcagaaaaaaaaaaagcctcctgcaaaagaaaaaaataaaataaaatgggggtccttctgaaattcccccagcctctcgatcctagctcatct
cagcccgccatttcgcttccattctaccaaaaatcacgggggggggaaccatcaggatcacagtattttgtaacccccactctgttagcaaatttgcgcc
cagggagtccttgcatggctttctggcaatcaaagactgacgtaaattctacaaagatcttgccgcatcctgggacttcaactccatccactggac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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