XB-CLONE-3233273
Clone Name: ptf1a-HAR413
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Gene:
ptf1a
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Species: Xenopus tropicalis
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Description: EXRC Clone Number HAR-413. IMAGE:7557710.
Xenopus tropicalis pancreas specific transcription factor, 1a. NOT Full length. Accession number CX496418.
To generate probe for in situ.
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Sequence:
5' EST: CX496418 [+]
cgctcgcacatccccactctgccctacgagaagcgactgtccaaggtagacaccctgcgtctggccataggctacatcaacttcctcagcgagttggtac
agtccgacctgcctctaaggaaccccaacaccgacagcggccatcagcccaagaaagtcatcatctgccaccggggcacaaggtccccatccccaagcga
ccccgactatgggctgcctccccttgcgggccattctctttcctggactgatgagaagcaactgagagaccaaaacgttgtcagaacggccaaagtgtgg
accccagaggaccccaggaaattaaacaaatcctcattcagcaacattgaaaacgaaccccctctgactttgtgccttgatatgtgactatttgagccgg
actgcttggtaccagtcggtaccgatgtgttaatatgtacataagacatgggaccatttaatttatagccctgctttttataattccaatggcaatcacc
acttgtgtatttatatatttattaccggtttgattaattgtaattgggatttgaacgtgtaatttatataatttaatgacatatgcaataagagcacctt
tttttgctacaacgatctaatttttattacaatattttggaagaaataaatctaatttattatttaaatgcaatctactaaggtttccgcgttgcgctct
atgtatttcatggcaggattagtcgctccttattgggtgggttttggggtccccttgacagaagggatgt
3' EST: CX496417 [+]atccccacaaactagaacctccttttctaggtaaaacatatcacagttacagctgcagtttcattaatttggccccatatatactcatgttttaattatc
cctacaaaaatggcgattaaccctctgtctcatctgccagtaacccaacttgcccctcacatttcctatataagctcaatatcccagaggagcagcagat
tggagggcccatagctgtacctatcagcactgcccctttacatggattgtagatttgtagccagaatatatccagatcttgtggaactacagctcccaga
atcccttgacatgagtttcgacggctgaggcgccctgaagcgcaaaggtatatatagcgctaaagcaatcacatgatagcgccggtataaggcggataac
cagccagcgtttctaaagctgcaacaatgaagcgaagcggactggatacactgagccttcagctcttgtacccggctcattacttgggcaaattgctcat
atctggcgcagactttgcgctgaggctcagttccagttagaacacgaatcccattgtgacatcccttctgtcaaggggaccccaaaacccacccaataag
gagcgactaatcctgccatgaaatacatagagcgcaacgcggaaaccttagtagattgcatttaaataataaattagatttatttcttccaaaatattgt
aataaaaattagatcgttgtagcaaaaaaaggtgctcttattgcatatgtcattaaattatataaattacacgttca
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Whole mount in situ hybridization:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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SalI |
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Suppliers:
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