XB-CLONE-3225988
Clone Name: sim1-865
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Gene:
sim1
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Species: Xenopus tropicalis
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Description: EXRC Clone Number 865.
Xenopus tropicalis single-minded homolog 1. Image EST 7563377. To generate probe for in situ.
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Sequence:
5' EST: CX468007 [+]
gttttcttatacaagctcaaatccaaccataaatgacagcagaaaaagcagtaaagctcgactctccaacacaaaatctaaatccagaacatcaccctac
ccacagtattctggattccatactgaacgatctgaatcagaccatgaaagtcaatggggcggtagccctcttactgacacagcttcaccacaacttcttg
atcccatagaaagaccaagctcccaccaacacgatgtatcttgtgcatatagacagtatccagaccgaagtggcctttgctttggattttccattgacca
ttcaaggttaagtgatgatagacaccaccatagtcagtcgtgtgaaggaggcagatgtgaggctggcagatactttcttggaagtcctcagactggaaga
gagacctggtggggttctcgatctgtgttgcctgttccaaagacctctccagatggaaaagacatatatgaggctaccatgccacacattgcatcaggtc
acagagcacatggaagaagtcattgggaagaagatagtgttgtcagttctcctgatccaggctctgcaagtgaatcaggggatcgttaccgcactgatca
atatcaaaatagtcatcatgaaccaagcaaaattgaaactcttatacgtgctacccagcagatgattaaagaggaagaaagcagaatacagctgcgcaaa
cctagtccagatcagctaactaccattaatggagctggtaaaagaaactctgtttgttttgcaaattttcaccagccacagttaactggagttgtgtgcc
gtggtccacctctatctaactcgtctccttgtgaccatgttcagcaacgggatggaaaaatccttagtcctcgtg
3' EST: CX468006 [+]catttatacacaccctgatacaggtcagagaaaatcatcaactcccgttactaataattactgatgtacccttgtgcacttgagcagaatcctgttgcat
ccttaaatgagaagtcacatcaaaatgtgttccgttacatcccaatgaatagttcctaatagattcactgtcataaagatgttcaagagcatacccagta
aaagtgtaagtatgtttgtcaaaatattgcctgtgagaactgtagtaatttggggatgcagaatggtccattgctggtgatggggacaaatttgcatgta
aataatcttttgctatggttacacttgattttgaaactctgtcagaatttggactactcattcttgacaaagcatttgggctgttttcatattcgtggtc
acgaggactaaggatttttccatcccgttgctgaacatggtcacaaggagacgagttagatagaggtggaccacggcacacaactccagttaactgtggc
tggtgaaaatttgcaaaacaaacagagtttcttttaccagctccattaatggtagttagctgatctggactaggtttgcgcagctgtattctgctttctt
cctctttaatcatctgctgggtagcacgtataagagtttcaattttgcttggttcatgatgactattttgatattgatcagtgcggtaacgatcccctga
ttcacttgcagagcctggatcaggagaactgacaacactatcttcttcccaatgacttcttccatgtgctctgtgacctgatgcaatgtgtggcat
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Whole mount in situ hybridization:
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Expressed in somite and muscle.
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
SalI |
EcoRI and ClaI can also be used. |
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Suppliers:
Search for clone at:
EXRC