XB-CLONE-3198151
Sequence:
5' EST: CX476029 [+]
aagaggggcacttccaggccagtccctccctcaaggagccaatgggaatgtgggaggaagcgagtgacccagtgatggggaagaaggataaaaatgagga
gcggaaggagagaatcctgaatctcacactggagattatctatctgctgactggagagggctacgtcatccctaagaagaagagcccaactgtgggtttg
ggggccctgcatgcccctggctccgtcatacagaaggaaaatgacaagaagatcctggaactcatctccaacatcatccagctgctgactggagaggagt
cggaaaatttacaaaaacacagttccataattacagaagggacaaaggaggaggaggctgtgcagctccacccactggcagcctgtgagtataaagatgg
gagcgatgttacagcacatacggaagcaactttatgttgtaataatgatgggaatctcacaaaccctgatgtttctccagcggaacagcccacaccggcc
aatgggattaaagaggaggcggcttcatgtgaagggggaaaccaatcagattgcagcattaatccccttacagaccagatacagggaacagacacaccta
ctcctatcatggggcggagcctgaataacagcttggcagataattatacatcgattgttattaa
3' EST: CX476028 [+]ccaaaatagatttaatataaagaaacttttatacagtaagaagcatattgtgggttgaggagaggcagagatttattccactttccatgaactgaggtcc
ctcagttcccaatggggagccagtgccgtgggaggagcattataaggcacctgacttagcatcacctgtgtccatcaggccccggagtcttcagctgaac
atgattggtctaaggattgtcctatggatacattcagttgtatataatgatgttcttctcctctgtaaatgtcaacatacatagtaggatcaatgggaaa
gaaatgttgcctgatgtcttatttgtaaaccatttcatccacttaaaaagcttacgggttttccccctttgtgactctaaaaatgtattttaagattact
gttacaagtaaaacatttcccacattcagtataggaaaatggcttctctcctgtgcagatctgttgcaaatatacagtaaattctgatgaactgacaaaa
catttaacaaaaggcacattgatataatcgtctttgatgggaattaagactatataaaatagcaaaacatttcccacattcagaacaagaatatggtttc
tcccctgtgtgggttttataatgaattttaaggttggatagctgtgaaatatttgcctgatgacaatttgtcaaacgatgacaacaagcctatggttttt
ctcctgtgtggctctgaaaatgtatcttaagattaccgttacgtgtaaaaccttttcc
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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