XB-CLONE-3198010
Clone Name: IMAGE:7535297
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Gene:
itsn1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157140 [+]
CGGACGCGTGGGGGGACGGCGGTGCGGGGTGAGGGGATCCGCACTGTACTACAGGCACAAGGGGAGAGTCGTACGGACACGGGCATTGGGAGAGTAGTAA
GGGGAGGAGGGGCGCTGAAGCAGAGGGCATGTTGCCATACAGCCGAGCTGTCTGAGAGGGGAGCCGTATTGGACATAGCCGGTGCTAATCCCTGTGGCAG
AGGAGAGGGTGGAGGACCGCTATGAGGCACCTACTAGCAAGCTAATATTGATACAACCATGGCTCAGTTTGGAACTCCTTTTGGGGGTAATTTGGACATC
TGGGCCATAACGGTGGAGGAGCGAGCTAAACATGACCAGCAGTTCCATGGGCTCCAGCCAACAGCTGGATATATCACTGGTGACCAAGCTAGGAATTTTT
TCCTTCAGTCCGGCCTCCCCCAGCCTGTGCTGGCACAGATATGGGCCCTGGCTGACATGAACAACGATGGGAGGATGGACCAACTGGAGTTCTCCATAGC
TATGAAATTGATCAAGCTAAAACTACAAGGTTACCCGCTGCCCTCTGCTCTCCCTTCCAATATGTTGAAGCAACCAGTTGCCATGTCCGCTGCTGCCGCC
GCTGCTGCCGCTGCGGGGTTTGCCACGTTGCCAAAGAGTTCTTCATTCGGCCGCTCAGGTGCCGGCTCCCAGATGAACACTAAACTTCAGAAAGCTCAGT
CCTTTGATGTCCCAACCCCCCCACCCCTGGCAGAATGGGCTGTACCACAGTCTTCAAGGTTGAAGTACAGACAGTTGTTCAACAGCCACGACAAAACCAT
GAGTGGGCATTTAACAGGTCCTCAAGCAAGAACTATTCTTATGCAGTCAAGTTTACCGCAGAGCCAGCTAGCTACGATATGGAACCTTTCAGACATTGAC
CTAGATGGAAAACTAACGGCCGAAGAATTTATTTTAGCTATGCACTTAATAGATGTGGCCATGTCTGGCCAGCCACTTCCTCCAGTTCTGCCTCCTGAGT
ATATTCCCCCATCTTTTAGAGTTCGATCGGGGAGCGGGTTATCGATTATGAGCTCGGTGTCTGTAGATCAGCGCTTACCGGAGGAACCAGCAGAAGAGGA
GCCACAGAACACAGACAAAAGACTTCCAGTGACATTTGAAGACAAGAAAAGGGAAAACTTTGAGCGAGGAAACCTGGAATTAGAAAAGAGGAGACAGGCA
CTTCTAGAGCAGCAGCGCAAAGAGCAGGAGAGGCTGGCACAGCTGGAAAGGGCAGAGCAGGAAAAAAAAAAAAAAA
5' EST: CX476164 [+]ggggacggcggtgcggggtgaggggatccgcactgtactacaggcacaaggggagagtcgtacggacacgggcattgggagagtagtaaggggaggaggg
gcgctgaagcagagggcatgttgccatacagccgagctgtctgagaggggagccgtattggacatagccggtgctaatccctgtggcagaggagagggtg
gaggaccgctatgaggcacctactagcaagctaatattgatacaaccatggctcagtttggaactccttttgggggtaatttggacatctgggccataac
ggtggaggagcgagctaaacatgaccagcagttccatgggctccagccaacagctggatatatcactggtgaccaagctaggaattttttccttcagtcc
ggcctcccccagcctgtgctggcacagatatgggccctggctgacatgaacaacgatgggaggatggaccaactggagttctccatagctatgaaattga
tcaagctaaaactacagggttacccgctgccctctgctctcccttccaatatgttgaagcaacccagttgccatggtccgctgctgccgccgctgctgcc
gctgcggggtttgccacgttgccaaag
3' EST: CX476163 [+]cctttttttttttttttcctgctctgccctttccagctgtgccagcctctcctgctctttgcgctgctgctctagaagtgcctgtctcctcttttctaat
tccaggtttcctcgctcaaagttttcccttttcttgtcttcaaatgtcactggaagtcttttgtctgtgttctgtggctcctcttctgctggttcctccg
gtaagcgctgatctacagacaccgagctcataatcgataacccgctccccgatcgaactctaaaagatgggggaatatactcaggaggcagaactggagg
aagtggctggccagacatggccacatctattaagtgcatagctaaaataaattcttcggccgttagttttccatctaggtcaatgtctgaaaggttccat
atcgtagctagctggctctgcggtaaacttgactgcataagaatagttcttgcttgaggacctgttaaatgcccactcatggttttgtcgtggctgttga
acaactgtctgtacttcaaccttgaagactgtggtacagcccattctgccaggggtgggggggttgggacatcaaaggactgagctttctgaagtttagt
gttcatctgggagccggcacctgagcggccgaatgaagaactctttggcaacgtggcaaaccccgcagcggcagcagcggcggcagcagcggacatggca
actggttgcttcaacatattggaagggagagcagagggcagcgggtaaccttgtagttttagcttgatcaatttcatagctatggagaactccagttggt
ccat
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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