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Summary Attributions
XB-CLONE-3192368

Clone Name: IMAGE:7886820 Gene: myh13 Species: Xenopus tropicalis  
 

Sequence:

5' EST: DT386327 [+]

3' EST: DT386326 [+]

Library:

Name: NIH_XGC_tropMet7External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library

Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis (1.5-2.5 kb fraction; NIH_XGC_tropMet8 contains the 0.5-1.5 kb fraction) and ligated into SalI and NotI digested pCMV-SPORT6 vector. Primary library, non-amplified. Library constructed at the DOE Joint Genome Institute.

Anatomy: whole organismStage: NF stage 62
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pCMV-SPORT6

Type: phagemid

5' Restriction Site: SalI

3' Restriction Site: NotI		Expression Image

Usage:

Suppliers:

Search for clone at: Open Biosystems