XB-CLONE-3191196
Clone Name: IMAGE:7849584
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Gene:
spon2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387063 [+]
taaatgtgcatcatcttatggaagaacctgtcaagcaggttgtattacagacagctggggaagtcagccttcaggaaggattctccttccaggcaatgaa
aatgtcttcttccaggcaagaggcctccttcagcagtatctccgagacaaagagcatgtcctcaaaaagtgaggcatcatttgttgaaatgagttcaagt
agctttatggaaaaatccagtctgatgcagagttcatctagcagaatgcttacagctggtttcagaggaatacctcccaaaattgaagctcttcccaatg
atgtcagcattgacgaaggaaaagtactaactgtggcttgtgccttttctggagaacccacaccagatattgcatggtctaaggctggacatccagttcc
cactcaagaacaaaaaggcagagttcacattgaaacttctgaagacttaaccactcttatcatcatggacattcagaagaaagatggcggacattacact
cttaacctgtcaaacgactttgggtccgattctgcatctgtgaatattaatgtccgaacacattaagcattttatcgtttacattcacatgtaattttac
aagtgattcactagaactagatgaactgatttgtaaatataaaagctatttttgtacctacctgtataagacaaagtcgaaaagatttacgctatcacat
attcctatttgaatagggaaattgatacatttttaactgacatgtacatactgtatatagccgagtttaccggttatggagttctgtaccatatatttta
tgacgttttaaaatgaaactggaaccaacggtgggcaggagaaagtttcctagggagcgaataccttgcctgccatt
3' EST: DT387062 [+]aatggtgcagaaaacatgctttattgcaatcaaagagctatcttcgggaggttcaataaataataatgctacaataacataaaataaaacaataaataat
gacgaagtgaagggcattcggaagttcttaacaacagcctgaaaagctgcggtgcatcactttattgccctgttttctcaacatataatatagtccctat
tctaccacacacaatcactgcacccgataaacctcctgaagttggcactgattctgcacttgagtaccttaacctgctttgttgagggtatggccaggtg
atgttactttaaagcaagctgagggagtcaatgtgtcagaaaaagtgcagagggttttttatgaacaaagtggccgcttgcactttgaacatacaaaagt
tttgtctctgtaaaattgtaaatttccattatctgtaaataatgtaaatatgtaggtgttgcctgcaggagggtagttgcaagcagcacctaatattcac
agtaattcaatccaaatacttggatttcgtatagttcgtgtagagcatcctctcttgggtgtaggtttgtcttcaacagggagtttctcaacctgctgag
gtttgcttagacatcaacgtagagttgaggcacatagtttaatggcaggcaaggtattcgctccctaggaaactttctcctgcccaccgttggttccagt
ttcattttaaaacgtcataaaatatatggtacagaactccataaccggtaaactcggctatatacagtatgtacatgtcagttaaaaatgtatcaatttc
cctattcaaataggaatatgtgatagcgtaaatcttttcgactttgtc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: