XB-CLONE-3191134
Clone Name: IMAGE:7849446
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Gene:
zmym2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387104 [+]
agtatttgagccttttgtccctccccttatggatttgtgcccacatgtcctgccnctttttgtcacatcagagagagatgggtttcagtttagagtctga
tgcaggttgaatttttgtcagcctacacattagtgatgtgcggttcacgaaaaacccgactcgcacccgacccaaaccctacccgacctggctttttccc
tttctccactctatttatagtcccgcgcctgccccgcaatgacatcacaaaaggggcggggcgtgcgtctataaattcacaagccggaaaagaaagccgt
cagtgttaggttgcgggcaggtaaggtgagcagaagagctaaacccgaacccacccgcaagtgattttgcgagactagcccgacaccgaggtcccgcggt
tatagtccctgcgtgtatgttttgaagtgtgggagggaactggattcccctaaggaacctacacagacacagtacattagttcatacttcccccagcagt
tttgtagaaatgatcataattaatgtatccattgtagcagcttgctctggtccagacttttgtaaatctgagggagtctgcttagcttctttggtcactt
cagagaaagctatccactaataaatcaaatgatgacatttataaagtcagcagtctgcttcactgagaaggggaatgaaaacaaattaatttaaaggaca
tgtaaacccaaaaaaataatcatttgcttttgaaagcagcatttacttaacttctggtaatgactttttaaacaatgtggcaaaaaccaatctcctccag
caacacaggtttgtcagtctgctgccttgtngtacattgtttcagcagtcag
3' EST: DT387103 [+]gataaaatgttgatactttaatgacattagtatacaagttacaattctacttttttgcctacagaataaatgtatgcaaacattattattgttgaagaaa
attgttagaattttgtgctacacaagaaaaaaaaaacatacagtaataaaaattgccagatgaactctgcagataacagttatcaaattatttacacaca
gaaatggtcaaacataattaccatcctgagctgaatgtcataaatatcaagaccaagctgtgaaagtatgttaccctctactccaatttacaatatttgg
attctttgctcattaataggacaacttggatcatttgttccagagcactctacttcctttcccaatgtaaaatcctgttcctgatatctagttaaaaaaa
aaaaaaagacaaaaagtatcctaggatcacatagtaagatgcaataaataagattagattaggcagaaatgcaaccatttagaccttccaccaagacacc
aaaatagacagtaactacacatgcccccttattcacctcagtacactatatagaaagaaactggttccttgaaaagcaaaaagtgtgttacgtcactaca
ggtttagaccaaacaagtcaatatttcaatatataactattatttatgcttcctggcccaaatctctagccctggtaaccagtacataaaaggcaatgag
cttgatagagcattgtctgtaccattcatatcaactgcaagcccaatgaaagtgcaccatcttgtaagcatttcctaatcaaaccacataaagccatggc
cattcaccacctgaaggcagacgtcaaatccattaaggcaatctgattggttcctgaagaaactatccctagtttgtgct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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