XB-CLONE-3191128
Clone Name: IMAGE:7849542
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Gene:
aldoa
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387108 [+]
catgcctcaccagtacccagcactgacccccgagcagaagaaggagctccatgacatcgccaaacgcattgtggccactggcaagggcatcctggcagcc
gatgagtccacaggcagcatcgctaagcgcttgagttccatcggggcagagaacaccgaggagaaccgccgcttataccgccagctgctcttcaccgccg
acgacagagtgaacccctgcattgggggcgtcatcaccttccacgagactctctaccacaagagcgacgatggcgtcgccttcaccgaagtcatcaagaa
aaaagggggtgtcgtaggcattaaggttgacaaaggtgtcgtccctctggctggaaccaacggtgaaaccacaactcaaggtctcgacggactgtccgag
cgctgcgcccagtacaagaaagatggcgctgactttgccaagtggcgctgcgtgctcaagatctcagaacacactccctctcatctggccatcattgaga
atgccaacgtattggctcgctatgccagcatttgccaacagaacggaattgtccccattgtggagcccgaggttctccctgatggagaccatgacctgaa
gaggtgccagtatgtgacagagaaggtcctggctgcagtgtacaaggctctcagcgaccaccacatctacctggaagggaccctgctgaaaccaaacatg
gttacccccgggcatgcctgcaccaagaagtacccccctcaggaggttgctatggcaacagtcaccgccctcaggcgcacagtgccccctgctgttggag
gaatcaccttcctgtccggaggtcagagtgaggaagaggccaccgtgcacctcaatgccatcaaccagtgccccctggatcaaccctggcccctgacct
3' EST: DT387107 [+]tttcttgagtgggtcagaaactgctttattgagggtttgttcagcagtgacacatagaacagtgactcagagccgagaatacagatacaggaggtgcacg
tagcccggagacagacagtcgctctctctcacactcacacacgtcattcacgccctctgctggactgtccaggaatcaccatagcaacgctcagtccgca
gtctctctctctctccagcaaggggaatggcttagctaatgttccgcaggcgggtctctacattggggaggggctaaccgctctgcaggggaggagcccc
tcactaggcaaatggaagcggccaccacagtgactaggaggagtaggaagtctagtaggcgtggtttgatacgaagagagactcgccggcagcagctccc
gcctctccgctggctacgtatttgccttggcatgccaggctgttggccagggcacgcttgacgtactcctcctgggccttcttggtgttttccttcttgc
ccccccaggctttgagggcggaggcctgaagggcacggccgtaggagaaggtcaggggccagggtttgatcagggggcactggttgatggcattgaggtg
cacggtggcctcttcctcactctgacctccggacaggaaggtgattcctccaacagcagggggcactgtgcgcctgagggcggtgactgttgccatagca
acctcctgaggggggtacttcttggtgcaggcatgcccgggggtaaccatgtttggtttcagcagggtcccttccaggtagatgtggtggtcgctgagag
ccttgtacactgcagccaggaccttctctgtcacatactggcacctcttcaggtcatggtctccatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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