XB-CLONE-3191104
Clone Name: IMAGE:7849544
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Gene:
c3h15orf40
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387124 [+]
attttttgtagccccacagaaaacaaatcgcttatattctcccccaggatggtgcgtctcttctgcaaaaaaaaaagcttgtggatgtcccagactgtgc
aaatatgaggtatagtaaaatgtcctgtttgttattgtacccatgtgctgatttcagtggtgcaatactgcacaagcacacagtccaggacataggaaat
agatcaatgggaattaaagatggaaaagtaaccttgtttggtatgtttaaagaagtggagcaccaggtgggggtgagaaaaaaaatcggctattcagtgg
ggtgggggatgtataataaattggctccctacccacccagtgattactactttcctatcctttattatgaattccttttcttaaaaatgtcctatggtta
gtgttgggtgtccatatttttctttccttgatatgtgcatacgcctttagcaagcacaagcaaaatatcacacatttacaagtgtgacttaaagggcttc
acttgccaactgctactgtaagtgtatgtatacacatgcatgggagtggaaggttcaagcgtatgatggacatttatttggctgttagaatttttttttt
ttttttgcatctaattgttaacaattagaatttgctgtttacaacaattttttgcttgtgttttcccccttttttctcagttttcagatttatacctgaa
gttataaaaggtttgtgctagttcttgtgaaaaatctgtcaaactgttgtatggcagtatattgtcacatgtaggaaatttagtttgcattgtgcaaatt
taaacaaaacttacactttataggtactgaagatgtttaggctcaatttagat
3' EST: DT387123 [+]ttcggcaggcataatttattaggaatattactagaaacagaagatgacattgagactatgtaataattgtaatattttatcttacataaagtgtaccact
ttgctggcttttgaaaatttacactgaattctttgagtggatttattatctaaattgagcctaaacatcttcagtacctataaagtgtaagttttgttta
aatttgcacaatgcaaactaaatttcctacatgtgacaatatactgccatacaacagtttgacagatttttcacaagaactagcacaaaccttttataac
ttcaggtataaatctgaaaactgagaaaaaagggggaaaacacaagcaaaaaattgttgtaaacagcaaattctaattgttaacaattagatgcaaaaaa
aaaaaaaaaattctaacagccaaataaatgtccatcatacgcttgaaccttccactcccatgcatgtgtatacatacacttacagtagcagttggcaagt
gaagccctttaagtcacacttgtaaatgtgtgatattttgcttgtgcttgctaaaggcgtatgcacatatcaaggaaagaaaaatatggacacccaacac
taaccataggacatttttaagaaaaggaattcataataaaggataggaaagtagtaatcactgggtgggtagggagccaatttattatacatcccccacc
ccactgaatagccgattttttttctcacccccacctggtgctccacttctttaaacataccanacaaggttacttttccatctttaattcccattgatct
atttcctatgtcctggactgtgtgcttgtgcagtattgcaccactgaaatcagcacatgggtacaataacaaacaggacattttactatacctcatattt
gcac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: