XB-CLONE-3191095
Clone Name: IMAGE:7849306
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Gene:
hnrnpd
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387130 [+]
cagtagtatacgttctacttgtccgacttattgttattaaaaacgtaagccatgatttctgaagacgatgaagccatgatggaggctactactgctgaga
cagaggagactgggcaaaacgaaggggtgaagattgatgctagtaagaccgaagaggatgaggggaagatgtttataggaggccttagctgggatactac
aaagaaagacctgaaagattatttttcaaaatttggagaggttgtagactgtaccttgaaattggaccctatcactggaagatctcgtggctttggcttt
gtattgtttaaggaatctgaaggcgttgacaaggtaatggaacagaaggagcacaagctgaatggtaaagttatagacccaaagagggcaaaggcaatga
agacaaaagagcctgtcaagaagatatttgtagggggattatctccagacacacctgaagagaagataagagagtattttggaacgttcggagagattga
agccatagaacttccaatggataacaagactaacaaaagacgtggattctgcttcatcacatttaaggaggaagacccagtaaagaagataatggaaaag
aaataccacaatgttggcctcagtaaatgtgaaattaaggtggcattgtcaaaggaacagtatcagcagcagcagcagtgggggacaaggggtggaggct
catcatctaggccccgtggaagaggaggtgtaagtcagacctggagccagggatatagtaactactggaatccaagctacagcagttatggatacaacaa
ccaggggtatggaggttatgggaactatgactactctgnngtacactactatggctatggagactatagtaatcagcag
3' EST: DT387129 [+]tttttttaaaattttaaaggaatatttattaaaggggaaaaaagcttacattatgacacaggcatacaaagttcataaacacaagatttcttcttccatg
ctggtgaataaagtgaacacaatttttaaacaagcaaagctattagaaaataagacagctaggtgaatcctataaaataattttcactacatcccttttt
aagggttttgcaaaaacaaacaaaaaaaaaaagggggggagggggggaagaaacataatttgcactgctatatgcatgcagaatgagccacctcaaatca
ttcttcaacccagaaaatactgcttgttcacctgtttatgacacgttgtgaattgaataatttagtatggtttgtaactattttgatgaccacctcgtct
ggctgttttcccataaccactctgctgattactatagtctccatagccatagtagttgtaaccagagtagtcatagttcccataacctccatacccctgg
ttgttgtatccataactgctgtagcttggattccagtagttactatatccctggctccaggtctgacttacacctcctcttccacggggcctagatgatg
agcctccaccccttgtcccccactgctgctgctgctgatactgttcctttgacaatgccaccttaatttcacatttactgaggccaacattgtggtattt
cttttccattatcttctttactgggtcttcctccttaaatgtgatgaagcagaatccacgtcttttgttagtcttgttatccattggaagttctatggct
tcaatctctccgaacgttccaaaatactctcttatcttctctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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