XB-CLONE-3190991
Clone Name: IMAGE:7849602
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Gene:
eef2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387198 [+]
gacgagggtggaccacggtaaatctactctgactgactcactggtatgcaaggctggtatcattgcttctgctagggctggtgagactcgttttacagat
accagaaaagatgaacaagagagatgtatcaccatcaaatcaacagcaatctctctcttctatgagctctctgagaacgacttggcttttatcaagcaaa
gcaaagatgggtgtggcttccttataaacttgattgactcccctggccatgtagacttttcctctgaagtaactgctgctcttcgtgtcacagatggtgc
cttagttgtagtggactgtgtttcaggcgtctgtgtgcagactgaaaccgtgcttcgtcaagccattgcagagcgcatccgccctgtgttgatgatgaac
aaaatggatcgtgctcttttggagctgcagttggaaccagaggagctctaccaaacttttcagcgcattgtggagaatgttaatgtgattatttccacct
acggtgaaggagagactggacccatgggaaatatcatgattgaccctgttcttggcacagttggttttggctctggtctgcatggctgggctttcacact
aaaacagtttgcagagatgtatgtcgcanaatttgcagcaaagggggaaggtcagttggctcctgcagaacgggccaaaaaagtggaggacatgatgaag
aagctgtggggtgataggtattttgaccctgcanatggaaagttcagcaagtctgcaacaaacgctgatggcaaaaaacttcctagaaccttctgccagc
ttgtattggatcaattttcaggtatttgatgcaatcatgaattttaagaaagaagaaactg
3' EST: DT387197 [+]ttttttaatggcaccaataagtcattcaggttattgcagtacatctttgctctacattctgccctcgttcctgtcaaccctcaaattcacatttacttcc
ccagccatccaagatgttttcagagagcagaagctacccatcaagaataaatagaaagttatgatcatgccaatgaaagattatattcagggttccaaca
gaatgttgcagggaatgctgacatcttttgggtatagttcatttccacttaatcaatgctgacaggagtaaagcaaaggtttacagcttgtcaaggaagt
tatccaaggcttgaacaccctctttcaagcccttcctcttccgggtctctgccaccacttggaaaggcctggtggagttatcgaaaggatctccaggtag
aatttgccagtggtcaaacacacactgagggaaggcctggccaccagtgtttgatcttaagtcagcagtgaaaccaaatgactcatttacaggcaggtat
gccttaaccacaaacattggtgttccagccacctgggattcttcaaagacatgcccacgctttctgttcaagacaccatagatacctcctaccacctgct
cagggcactgaatttcaacaaggtagattggttccatcagacgtggctgagcagtcaacacacaagcatacaagacgcggcgtgcagttggaatgatctg
tcctcctcctctatggatggcatcagcatgaagtgttacatcatggacatcaaaacgaacacctctcanattttcttcacacaggacaccctcctttgta
gcccactggaatcctgncacaacgctgtctttgatttcattcagatactgaactcccttagtaacatcagtaagtatgttgggcccagagccat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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