XB-CLONE-3190952
Clone Name: IMAGE:7849510
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Gene:
aldoa
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387224 [+]
tttgttcagcagtgacacatagaacagtgactcagagccgagaatacagatacaggaggtgcacgtagcccggagacagacagtcgctctctctcacact
cacacacgtcattcacgccctctgctggactgtccaggaatcaccatagcaacgctcagtccgcagtctctctctccagcaaggggaatggcttagctaa
tgttccgcaggcgggtctctacattggggaggggctaaccgctctgcaggggaggagcccctcactaggcaaatggaagcggccaccacagtgactagga
ggagtaggaagtctagtaggcgtggtttgatacgaagagagactcgccggcagcagctcccgcctctccgctggctacgtatttgccttggcatgccagg
ctgttggccagggcacgcttgacgtactcctcctgggccttcttggtgttttccttcttgcccccccaggctttgagggcggaggcctgaagggcacggc
cgtaggagaaggtcaggggccagggtttgatcagggggcactggttgatggcattgaggtgcacggtggcctcttcctcactctgacctccggacaggaa
ggtgattcctccaacagcagggggcactgtgcgcctgagggcggtgactgttgccatagcaacctcctgaggggggtacttcttggtgcaggcgtgcccg
gnggtaaccatgtttggtttcagcagggtcccttccaggtagatgtggtggtcgctgagagccttgtacactgcagccaggaccttctctgtcacatact
ggcacctcttcaggtcatggtctccatcagggagaacctcgggctccacatggggacaattccgttctgntggcaatgctggcatagcgagccaatacgt
tggcattctcaatgatgg
3' EST: DT387223 [+]cagcactgacccccgagcagaagaaggagctccatgacatcgccaaacgcattgtggccactggcaagggcatcctggcagccgatgagtccacaggcag
catcgctaagcgcttgagttccatcggggcagagaacaccgaggagaaccgccgcttataccgccagctgctcttcaccgccgacgacagagtgaacccc
tgcattgggggcgtcatcaccttccacgagactctctaccacaagagcgacgatggcgtcgccttcaccgaagtcatcaagaaaaaagggggtgtcgtag
gcattaaggttgacaaaggtgtcgtccctctggctggaaccaacggtgaaaccacaactcaaggtctcgacggactgtccgagcgctgcgcccagtacaa
gaaagatggcgctgactttgccaagtggcgctgcgtgctcaagatctcagaacacactccctctcatctggccatcattgagaatgccaacgtattggct
cgctatgccagcatttgccaacagaacggaattgtccccattgtggagcccgaggttctccctgatggagaccatgacctgaagaggtgccagtatgtga
cagagaaggtcctggctgcagtgtacaaggctctcagcgaccaccacatctacctggaagggaccctgctgaaaccaaacatggttacccccgggcacgc
ctgcaccaagaagtacccccctcaggaggttgctatggcaacagtcaccgccctcaggcgcacagtgccccctgctgttggaggaatcaccttcctgtcc
ggaggtcagagtgaggaagaggccaccgtgcacctcaatgccatcaaccagtgccccctgatcaaaccctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: