XB-CLONE-3190552
Clone Name: IMAGE:7849737
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387488 [+]
ctggatcttcctactacatttctgagattatcccacccacatccattacttatgtattcccctgtagatggccattttttgacctctaatgatcttaatg
gggtctcctgtcccctttgatggtggtaagggttagggtgtttaggctctgtctctagcccctgtcatctacttctgtgctggcatcatatcatcaatgg
actgtcccttctcaagcttcttctccatctcaatcatgagtttcacaccatccactaccatctggacttggtccacttcagagaagcccagcctatcagc
attggagatatcaaagacacctcctactgctgcagtgtcaacaccacctgtgcctctcttctgcagacgaagcctgcccagtatctcatcaaatttgggg
tgtttgctgaggttggggagcttggcatggacacctcctctcaggcctgtgcccaagtttgatgggcaggtaaggacataacccagatgttcattccaca
tgaaggggtgtcctgcttgtttaaagatctcttcaatcttctgaagtccctcacagaagcgtctgaagacttctttcatgtttcctcctttctgcatgga
aatgactctcagatggtcctcttcatttacccacaccaggaaggtcttgttttcattgtgccagataccacgggcatcaggccagtcacggcccatgcca
gaggccagaagcagaggggacactggtttgtcaaacaggaagtggtcatcaatcagctgctgctgctctgcatctgacatatccttg
3' EST: DT387487 [+]gcaggtctagcccgatcttccacatgcctttcggtaacacccataataactggaaattgaattattcctctgaggaggaatatcctgacctcaccaagca
caacaatcatatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacaccaagtggcttcaccttggatgatgtcattcagact
ggagttgacaacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcctatgaggtgttcaaggacctttttgatcccatta
ttgaggatcgtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaaccttaagggaggtgatgaccttgacccaaactatgt
gttgagcagccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgtggggagcgtcgtgccattgaaaaacactccatc
aaagctctcagcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatgcagagcagcagcagctgattgatgaccacttcc
tgtttgacaaaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgatgcccgtggtatctggcacaatgaaaacaagaccttcct
ggtgtgggtaaatgaagaggaccatctgagagtcatttccatgcagaaaggaggaaacatgaaagaagtcttcagacgcttctgtgagggacttcagaag
attgaagagatctttaaacaagcaggacaccccttcatgtggaatgaacatctggggtatgtccttacctgcccatcaaacttgggcacaggcctgagaa
gaggtgtccatgccaagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: