XB-CLONE-3190451
Clone Name: IMAGE:7849889
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Gene:
rab14
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387555 [+]
ccaggctccgcaccgtccgacaccgctcccactcagaatcctctttggcgtttgtgaatagcggcccggaccttcgtcccttgcacgcgccaccatggca
accgcaccttacaattactcttacatctttaaatacatcattatcggtgatatgggagtagggaagtcctgcctgctgcaccagttcacagaaaagaaat
ttatggccgactgcccccatacgatcggcgtggaattcggcacccggatcatcgaagtcagcgggcagaaaatcaaactgcagatctgggacacagcggg
gcaggagcggttccgggccgtcacgcggagctattacagaggcgcggccggggcacttatggtctatgacatcactaggagaagcacatacaatcattta
agcagttggctgacagatgcacggaacctcaccaatccaaatacggttatcatccttataggaaacaaagcagatctagaagctcagagggacgttacct
acgaggaggcaaagcagtttgctgaagagaatggtctcctgttccttgaagcaagtgcaaaaacaggggagaatgtggaagacgcattcctggaggcagc
caaaaagatctaccagaacatccaggacgggagcctcgatctgaacgcagcggaatctggcgtacaacacaaaccgtccgctccccagggcggccgacta
accagcgagccccaaccccagagagaaggctgcggctgttagtgacgtcccccccccccttcctacccccggcggtggcagttcgtttcttgaccttccc
ccgctctgtctgtcccaagcagtattttgttactgccccattatcttctgtacattctattgg
3' EST: DT387554 [+]aaagcacaaaaaagaaaaatatatatattctatgaatgattaagacagacgttgttggaaaccccgccctcccgccgcataataaggctgatggctcctc
ctactgcacggagacgcaattccaggcaacgtctgggggaggggctaggagcgccatcagtcttgcactgcggttacagaaaccccgcccccgcgtgaac
tgattcacagcaaataaacctacagagtatgacgactcacggaaaggaaaggggggcgttacttacaataagaggtggagcttgtaagacggcctggcga
cgaatcagataagtccagcatcagtgctatgttaaaatcatggaacatcacaaatacgattaagaaaaaaaaaaagttctagattttaccatgcaggaaa
ggggtttttttttgttttttgttttttctgtgtgtgttttgtgtttatttactcgccgggggaataacctgaatacatctagttatttagcagtttagta
tcgggttaaactgtcgtcttagcagagccccgccctcccacctttaatttaaaccaatagaatgtacagaagataatggggcagtaacaaaatactgctt
gggacagacagagcgggggaaggtcaagaaacgaactgccaccgccgggggtaggaagggggggggggacgtcactaacagccgcagccttctctctggg
gttggggctcgctggttagtcggccgccctggggagcggacggtttgtgttgta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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