XB-CLONE-3190412
Clone Name: IMAGE:7849749
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Gene:
ppip5k1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387581 [+]
cttcctcttgttaagtcttgagcatcatatagtatggctgcatgaatcagtaatggtaccaagtgatatgttgttgagaagataagttggaccatgtcac
ataatcagaggaagataataaaaacaacgaaagatggttttataggtgtggcattttcgcatacagaaatatagaaattattactgagtgatcagcaaaa
agtgttgtatcttttaatgttgaagaaccctatttggaaacattaaatgtccctcttcccccccttttttatttttaatcttttccaggttttgagggtt
gttcaatggtacctactatctaccctctggagacacttcacaactcactgtccctcaggcaggtcaatagcttcttgacagcagtttgtaaacagtttgc
agatacccattcaagaagatcttcagctgggtgtgactccatgatttccagccatacatctggtgacacttacttgccacatcgaatcctttcttcttct
tcacttcattatcgtcaccgctcagagaggcctccctggtacagcagcggcccatctagcacagtgtccagtgcagggccatcgtccccaactgctttag
atctttcagctcgttttaacttcagtgataaaacatcccttactgaagagcaaaggaaaaaactaaaccttgaagagaatctacaggttcagaagtcaac
tggtgagggacaagccagcaaccaggaaattcatcagttggaggaaccgcttcatagcgattgcacatatttgccaccagagccttccagtgatgcggct
gcacccgtgcaacggtgtgtagagcacgagcttcagcttggcatagagtcacttggagaagcttctgttcc
3' EST: DT387580 [+]tttgttcagttttgttacagagatgtctccctaccttgaagggctacttctgttatcatctgctcatctacaggagactgttctaggtgtaacatttgat
tatgggtttttgtgctctgggctgaaagctctgatagtgcttgaccaggaacagaagcttctccaagtgactctatgccaagctgaagctcgtgctctac
acaccgttgcacgggtgcagccgcatcactggaaggctctggtggcaaatatgtgcaatcgctatgaagcggttcctccaactgatgaatttcctggttg
ctggcttgtccctcaccagttgacttctgaacctgtagattctcttcaaggtttagttttttcctttgctcttcagtaagggatgttttatcactgaagt
taaaacgagctgaaagatctaaagcagttggggacgatggccctgcactggacactgtgctagatgggccgctgctgtaccagggaggcctctctgagcg
gtgacgataatgaagtgaagaagaagaaaggattcgatgtggcaagtaagtgtcaccagatgtatggctggaaatcatggagtcacacccagctgaagat
cttcttgaatgggtatctgcaaactgtttacaaactgctgtcaagaagctattgacctgcctgagggacagtgagttgtgaagtgtctccagagggtaga
tagtaggtaccattgaacaaccctcaaaacctggaaaagattaaaaataaaaaagggggggaagagggacatttaatgtttccaaatagggttcttcaac
attaaaagatacaacactttttgctgatcactcagtaataatttctatatttctgtatgcgaaaatgccacacctataaaacc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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