XB-CLONE-3190222
Clone Name: IMAGE:7849862
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Gene:
spon2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387707 [+]
agaacttccatggacaatgctctgccacagcatccttaaatgtgcatcatcttatggaagaacctgtcaagcaggttgtattacagacagctggggaagt
cagccttcaggaaggattctccttccaggcaatgaaaatgtcttcttccaggcaagaggcctccttcagcagtatctccgagacaaagagcatgtcctca
aaaagtgaggcatcatttgttgaaatgagttcaagtagctttatggaaaaatccagtctgatgcagagttcatctagcagaatgcttacagctggtttca
gaggaatacctcccaaaattgaagctcttcccaatgatgtcagcattgacgaaggaaaagtactaactgtggcttgtgccttttctggagaacccacacc
agatattgcatggtctaaggctggacatccagttcccactcaagaacaaaaaggcagagttcacattgaaacttctgaagacttaaccactcttatcatc
atggacattcagaagaaagatggcggacattacactcttaacctgtcaaacgactttgggtccgattctgcatctgtgaatattaatgtccgaacacatt
aagcattttatcgtttacattcacatgtaattttacaagtgattcactagaactagatgaactgatttgtaaatataaaagctatttttgtacctacctg
tataagacaaagtcgaaaagatttacgctatcacatattcctatttgaatagggaaattgatacatttttaactgacatgtacatactgtatatagccga
gtttaccggttatggagttctgtaccatatattttatgacgttttaaaatgaaactggaac
3' EST: DT387706 [+]ggtgcagaaaacatgctttattgcaatcaaagagctatcttcgggaggttcaataaataataatgctacaataacataaaataaaacaataaataatgac
gaagtgaagggcattcggaagttcttaacaacagcctgaaaagctgcggtgcatcactttattgccctgttttctcaacatataatatagtccctattct
accacacacaatcactgcacccgataaacctcctgaagttggcactgattctgcacttgagtaccttaacctgctttgttgagggtatggccaggtgatg
ttactttaaagcaagctgagggagtcaatgtgtcagaaaaagtgcagagggttttttatgaacaaagtgctggccgcttgcactttgaacatacaaaagt
tttgtctctgtaaaattgtaaatttccattatctgtaaataatgtaaatatgtaggtgttgcctgcaggagggtagttgcaagcagcacctaatattcac
agtaattcaatccaaatacttggatttcgtatagttcgtgtagagcatcctctcttgggtgtaggtttgtcttcaacagggagtttctcaacctgctgag
gtttgcttagacatcaacgtagagttgaggcacatagtttaatggcaggcaaggtattcgctccctaggaaactttctcctgcccaccgttggttccagt
ttcattttaaaacgtcataaaatatatggtacagaactccataaccggtaaactcggctatatacagtatgtacatgtcagttaaaaatgtatcaatttc
cctattcaataggaatatgtgatagcgtaaatcttttcgactttgtcttatacaggtaggtacaaaaatagcttttatatttacaaatcagttcatct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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