XB-CLONE-3190036
Clone Name: IMAGE:7849974
|
Gene:
erich1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC158312 [+]
ataatggcttagtaggctaagagaacagcttcattcaagcttctggtacattgaagaggtgactggggatacacatttaaagtgtttggggaggctgtgg
aagccttaggatgtcctccaacaggagaaatgtctttgtttccaatgttctgaaacgactgtaccctgaggaagacatttcagggtgcactcccagggtg
catggtgcaaagcctcccccagagagcaaggccaaggctgcttccagccaggaagacactgtacaagttcaggttgtgttcccaccagaggaattgttaa
aagcccccaaggtttacacggtgcttccacctccagaaggctatgtagcaagttctcagcatgagagcaaatgttcatcaccatcatcctcaaatgatga
agataccagagacgaagacttgaatacaacacgtaaaaggcgcagaaggaagaaaaacaagaaatctagcaatttgttaaaccctgaaatggagaggaat
tccactcaggccgagacaccttggcttgcatctgatcggagcgccattagcaaaagcaagaaaagaaagctgcagagaaagcgccagaaggaacgcacca
aggccactagaacggatggtacagatccaccaagtaaaggtgattccgaaggagttgataaagtgaaagacaccggtggggcagcttcagaagaagatca
aagagacaaggccaaggatctaatggaattccttcaggaaacacaagaaatttatctcatggatagatgtgcagattcggccgtgtccattcatggtatc
ctggacattttaggccggatggaggccggtgactttccatcatcagatgtgactctcttacatcaagtcaagactctcttgcttctgcaggacaccgaca
ggtcagaagaggctttggacaacttcaaagcaagttcctctctgcccgctgatcagagattagccatctattctctgttccgttactggattatccatat
tttgcccttgagaagaaaagaatagactttttaaaaaagactttttagaataaaaatatctgtgttcgttaatgaagaaaatgccaataaaagaacattc
attcataaaaaaaaaaaaaaaaaa
5' EST: DT387830 [+]ataatggcttagtaggctaagagaacagcttcattcaagcttctggtacattgaagaggtgactggggatacacatttaaagtgtttggggaggctgtgg
aagccttaggatgtcctccaacaggagaaatgtctttgtttccaatgttctgaaacgactgtaccctgaggaagacatttcagggtgcactcccagggtg
catggtgcaaagcctcccccagagagcaaggccaaggctgcttccagccaggaagacactgtacaagttcaggttgtgttcccaccagaggaattgttaa
aagcccccaaggtttacacggtgcttccacctccagaaggctatgtagcaagttctcagcatgagagcaaatgttcatcaccatcatcctcaaatgatga
agataccagagacgaagacttgaatacaacacgtaaaaggcgcagaaggaagaaaaacaagaaatctagcaatttgttaaaccctgaaatggagaggaat
tccactcaggccgagacaccttggcttgcatctgatcggagcgccattagcaaaagcaagaaaagaaagctgcagagaaagcgccagaaggaacgcacca
aggccactagaacggatggtacagatccaccaagtaaaggtgattccgaaggagttgataaagtgaaagacaccggtggggcagcttcagaagaagatca
aagagacaaggccaaggatctaatggaattccttcaggaaacacaagaaatttatctcatggatagatgtgcagattcggccgtgtccattcatggtatc
ctggacattttaggccggatggaggccggtgactttccatcatcagatgtgactctcttacatca
3' EST: DT387829 [+]atgaatgaatgttcttttattggcattttcttcattaacgaacacagatatttttattctaaaaagtcttttttaaaaagtctattcttttcttctcaag
ggcaaaatatggataatccagtaacggaacagagaatagatggctaatctctgatcagcgggcagagaggaacttgctttgaagttgtccaaagcctctt
ctgacctgtcggtgtcctgcagaagcaagagagtcttgacttgatgtaagagagtcacatctgatgatggaaagtcaccggcctccatccggcctaaaat
gtccaggataccatgaatggacacggccgaatctgcacatctatccatgagataaatttcttgtgtttcctgaaggaattccattagatccttggccttg
tctctttgatcttcttctgaagctgccccaccggtgtctttcactttatcaactccttcggaatcacctttacttggtggatctgtaccatccgttctag
tggccttggtgcgttccttctggcgctttctctgcagctttcttttcttgcttttgctaatggcgctccgatcagatgcaagccaaggtgtctcggcctg
agtggaattcctctccatttcagggtttaacaaattgctagatttcttgtttttcttccttctgcgccttttacgtgttgtattcaagtcttcgtctctg
gtatcttcatcatttgaggatgatggtgatgaacatttgctctcatgctgagaacttgctacatagccttctggaggtggaagcaccgtgtaaacct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: