XB-CLONE-3189976
Clone Name: IMAGE:7849788
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Gene:
spon2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387870 [+]
ccagccagaagacagtggaacataccgtgtagtgtgctcaaactacaaaggggaaacctctgactatgcaacattagacattgccggaggagatttttca
tcatatgtgcctaagcgcagagatgatattgttcctaagtcagcctttcctgaattattaaagtcagaagactatgccgtctcatctttcaagaaaactt
ccgcaatggaagctagctctgcaagaagagaggttagttcacaagtaacatctttaagagaatctagagagtcatatgaggcttatgcatctgaagagaa
gcgagtttctgcctctgaagccaaagctgtggaagaaagagttgttcacaggaagtttaagtcttcagagccagcaaaactactgacaaggccacagtca
atcacagttgctgaaggtgacagtgctagattttcatgtgactttgatggtgagccagctccaacagttacatgggcacatgctggacaaacaatctttt
cttctgtgcgtcatcaagtgacatcaacaaaatacaagtcaacatttgaaattacctctgtcaactcttttgatgaaggcagctacacagtagtagttga
gaattctgaaggaaagcaagaagcacattttactttgacaattaaacgtggaaaggcagcagaaaagggtgttacatccccatctagagtaaaatctcca
gagccacacaaaanatcccatgagtctataaagtcaccaaaaagagtgaaatcccctgaaccaactacaggttcaaaagctaaaccatctagcaagtcgc
ctgcagataaagtacagctacccacagttgcagctccaaagattgctcagaatctaaagttgcagcaagtgatgataaagtaaaaatgtcctgcatagtt
gaaag
3' EST: DT387869 [+]aagggggcagaaaacatgctttattgcaatcaaagagctatcttcgggaggttcaataaataataatgctacaataacataaaataaaacaataaataat
gacgaagtgaagggcattcggaagttcttaacaacagcctgaaaagctgcggtgcatcactttattgccctgttttctcaacatataatatagtccctat
tctaccacacacaatcactgcacccgataaacctcctgaagttggcactgattctgcacttgagtaccttaacctgctttgttgagggtatggccaggtg
atgttactttaaagcaagctgagggagtcaatgtgtcaaaaaaagtgcagagggttttttatgaacaaagtgctggccgcttgcactttgaacatacaaa
agttttgtctctgtaaaattgtaaatttccattatctgtaaataatgtaaatatgtaggtgttgcctgcaggagggtagttgcaagcagcacctaatatt
cacagtaattcaatccaaatacttggatttcgtatagttcgtgtagagcatcctctcttgggtgtaggtttgtcttcaacagggagtttctcaacctgct
gaggtttgcttagacatcaacgtagagttgaggcacatagtttaatggcaggcaaggtattcgctccctaggaaactttctcctgcccaccgttggttcc
agtttcattttaaaacgtcataaaatatatggtacagaactccataaccggtaaactcggctatatacagtatgtacatgtcagttaaaaatgtatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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