XB-CLONE-3189973
Clone Name: IMAGE:7849836
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Gene:
sod2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387872 [+]
acttgcacttgaccgcagacatgctgtgcaggctcagtgtttgtggcaggggaaggcttagatgtgctccagccttgacatactttacgtccagggaaaa
gcatactctgcctgacttaccatatgattatggtgctctgcagccccacatcagtgctgaaatcatgcaactacaccacagtaaacaccatgctacttat
gtgaacaaccttaatattacagaagaaaaatatgcagaggccctggctaaaggggatgttacaactcaagtctctcttcaagctgcacttaaatttaatg
gtggtggccatatcaatcacacaatcttctggacaaacctttcccctaatggtggaggggaaccacaaggagaacttctagatgccataaagagagactt
tggctcctttgagaaatttaaggagaaattaagtactgtgtcagttggagtccagggatcagggtggggatggttgggttataacaaggaatctaataga
cttcaactggctgcctgtgccaatcaagaccctctacaaggaacaacaggtcttatacctctgcttggaattgatgtgtgggagcatgcttattacttgc
aatataaaaacgttagaccagactatatgaaagcgatctggaatgttatcaactgggagaatgtagccgaaagataccgggcttccaaaaaataaagtgc
tagatctgttctctgttctgtccgttacagccagtgtaacacatctatacagcatctatagttaaagattatctgtctgttttcaaagagaaattgtgct
tctcacacatccctcagactgaatgcacttactagacttacaggccacgtccaatctgtgagcacttagaagcctgtagaaatca
3' EST: DT387871 [+]tttttttacattgctgctgcctttattattgaattgcaaaatgttattttcacaacagaccaccagtaaagctcaagctatgaaaaggattgattctaca
ggcttctaagtgctcacagattggacgtggcctgtaagtctagtaagtgcattcagtctgagggatgtgtgagaagcaacaattctctttgaaaacagac
agataatctttaactatagatgctgtatagatgtgttacactggctgtaacggacagaacagagaacagatctagcactttattttttggaagcccggta
tctttcggctacattctcccagttgataacattccagatcgctttcatatagtctggtctaacgtttttatattgcaagtaataagcatgctcccacaca
tcaattccaagcagaggtataagacctgttgttccttgtagagggtcttgattggcacaggcagccagttgaagtctattagattccttgttataaccca
accatccccaccctgatccctggactccaactgacacagtacttaatttctccttaaatttctcaaaggagccaaagtctctctttatggcatctagaag
ttctccttgtggttcccctccaccattaggggaaaggtttgtccagaagattgtgtgattgatatggccaccaccattaaatttaagtgcagcttgaaga
gagacttgagttgtaacatcccctttagccagggcctctgcatatttttcttctgtaatattaaggttgttcacataagtagcatggtgtttactgtggt
gtagttgcatgatttcagcactgatgtggggctgcagagcaccataatcatatggtaagtcaggcagagtatgcttttccctggacgtaaagtatgtcaa
ggct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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