XB-CLONE-3189916
Clone Name: IMAGE:7849984
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Gene:
arl6ip5
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT387910 [+]
attcagtctatggaatctccatgcagtttaggggagggcatccatctgggcactcagattaatggttacagttatgtccctgctcaggattatagggcca
aatacaaattagctgtgcctgcatgtgagtggcactttacggcagtgctgtgaaatgggggctaaatcacccttatggctgtattatatattctcataca
ttgggctgtcaataataccttaaggtgagctacagcccacatgtagacagcactgctgtgaggatttacacataatggcaaggtaatgtgcaccccaaca
tctggaactttaaacctgagctaataatggggatggagggttgcctcattgtaacattattatcccaaatatatctgtgccgctgtgagaattgggactt
gtctgatctgattagggacggaccagtaggacacacggtgtctgtagtgagaatcattctgagatagacctgagcttttgtttagattttctagaaatga
caaaagggtataaatcgtttttgggttttttttcttatcccacattgttgtaattccgtagatatagtatagctttcaggacaagactgcattggctgct
ttggcattgacattgcttttcgactgtttgcttcgagggtttccccctctcctttgcacatttgcacgcgttctgatccatgtattgtctcgttagtaga
cggggcaaactgctaaatgattccaagccaggaatgtccgaccctttctgagaggccgagggctggaagttgtattattggagcatctatgaagctacag
gttgcaacctgctctaagcttagctcaagactagac
3' EST: DT387909 [+]tttttttcgccattcagaatgtcagtttattaatattaaatgcttgttttccccagatacaatgctcccatacacaactggggaaacggcacgattactg
aacatgcattactatcagtcaggaatgtcggtgattctctggtctggaacagcagtgcaaaaactctgaatagtcaaacagaagatgcattttatgttgc
ggggtgagcctggacgtatgttgtgaagatataaaaagtcgaatatcatccaaaccggttataaatgtatctaaaaatctgagctgtcaatcacacattt
cctgccccgcctctatgccgaaggcatagaggcggggcaggcaacacacgattgtcagctcagatttttaaccacaattactccaactttgcattcagca
cttccaagatattacctagatgacataggcataagattttggggtaatacaaagtttcccttaataacagtgtacacaaaatggcgccggcctgctggct
gtgactgtaaatcccaggactgaaggggaaaaaaaattaaatactttatacagtgtaagtaaagtttattttgctcaacgaacatgatagaaaaggattt
ggaattattttttagggtaacaggtcccctttaattctaggggcaaacccaccaatcagctcattgcgctgccagcaactcaagggactatgcatatagc
actgagttggttcttcttggtacataaggaggcacacgggggtggggtgaataatgtttctacccaaaaacatatttttagcaaccaaaaaaggagctgg
ggaactcatagattaatatangtcttagttgttatt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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