XB-CLONE-3189670
Clone Name: IMAGE:7850007
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Gene:
gpx7
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121615 [+]
ATTAGGTGCCATGTATCTAACAGCTCTTGTTCTGCTTCTCTTGTTGCCCCCCTCACTGCAGAAATCCCGGGATTTCTATACATTTAAGGTGGTAAATATC
AGGGGCAAGCTGGTGTCGCTGGAGAAGTACCGTGGTTCGGTAACCCTGGTGGTTAATGTAGCAAGTGAGTGTGGATTTACAGATTCTCACTACAAAGCAT
TACAGCAGCTGCAGAGGGACCTGGGATCTTACCACTTCAATGTTTTGGCATTTCCATGCAACCAGTTTGGACAGCAAGAACCAAATAGTGACCGAGAAAT
TGAGAACTTTGTCAGAAAGAATTATAGTGCATCCTTTCCCATGTTCAGCAAAACTGCTGTCACTGGCACTGGTGTCAACAGTGCCTTCAAGTACCTAATA
GAATCCTCTGGAAAAGAACCGGACTGGAACTTCTGGAAATACTTGGTTGGGCCGGATGGTAAAGTAGTTGATGCTTGGGGTCCATCAGTCTCTGTAGCAG
AGGTGAGGCCTCACATCACCAGCCTTGTGAGGAAAATAATTCTGAAAAGGAAAGAGGAACTTTGAGACCACTTCCTAGACAAGTGCCTGAAATCTTATGG
ATCATTCCTTTCATTTGTTTTCAAGATATCAGTATGAAAATGCATAGAACATTTGGATATTGAGAATAATAAGTGAGGTATTCCCGTGACTCTGCAGGTG
TTTTATAGTAAAGTTTCATATTACACATATTACAGTGAAATAACTTGTCATGTCTCAGGAAACCACTGTGTAAAAAAAAAACCAATGCATGACAAACACA
AGCAAATATACCATAGTCTCTTTTTTTTAAAAAAAAATGTTTTCTGGCAATTATTACTTAGTTTACACTGTAATCGCCCAGCTGAAAAACACATTGTTCT
TATTGCCAAGTCATTGTTTGTATGTTGTGTCATGAATGAAATTTTGAAATTCAAAAAAAAAAAAAAAAAA
5' EST: DT388073 [+]attaggtgccatgtatctaacagctcttgttctgcttctcttgttgcccccctcactgcagaaatcccgggatttctatacatttaaggtggtaaatatc
aggggcaagctggtgtcgctggagaagtaccgtggttcggtaaccctggtggttaatgtagcaagtgagtgtggatttacagattctcactacaaagcat
tacagcagctgcagagggacctgggatcttaccacttcaatgttttggcatttccatgcaaccagtttggacagcaagaaccaaatagtgaccgagaaat
tgagaactttgtcagaaagaattatagtgcatcctttcccatgttcagcaaaactgctgtcactggcactggtgtcaacagtgccttcaagtacctaata
gaatcctctggaaaagaaccggactggaacttctggaaatacttggttgggccggatggtaaagtagttgatgcttggggtccatcagtctctgtagcag
aggtgaggcctcacatcaccagccttgtgaggaaaataattctgaaaaggaaagaggaactttgagaccacttcctagacaagtgcctgaaatcttatgg
atcattcctttcatttgttttcaagatatcagtatgaaaatgcatagaacatttggatattgagaataataagtgaggtattcccgtgactctgcaggtg
ttttatagtaaagtttcatattacacatattacagtgaaataacttgtcatgtctcaggaaaccactgtgtaaaanaaaaccaatgcatgacaaacacaa
gcaaatataccatagtctcttttttttaaaaaaaaatgttttctggcaattattactttagttacactgtaatcgcccagct
3' EST: DT388072 [+]gaatttcaaaatttcattcatgacacaacatacaaacaatgacttggcaataagaacaatgtgtttttcagctgggcgattacagtgtaaactaagtaat
aattgccagaaaacatttttttttaaaaaaaagagactatggtatatttgcttgtgtttgtcatgcattggttttttttttacacagtggtttcctgaga
catgacaagttatttcactgtaatatgtgtaatatgaaactttactataaaacacctgcagagtcacgggaatacctcacttattattctcaatatccaa
atgttctatgcattttcatactgatatcttgaaaacaaatgaaaggaatgatccataagatttcaggcacttgtctaggaagtggtctcaaagttcctct
ttccttttcagaattattttcctcacaaggctggtgatgtgaggcctcacctctgctacagagactgatggaccccaagcatcaactactttaccatccg
gcccaaccaagtatttccagaagttccagtccggttcttttccagaggattctattaggtacttgaaggcactgttgacaccagtgccagtgacagcagt
tttgctgaacatgggaaaggatgcactataattctttctgacaaagttctcaatttctcggtcactatttggttcttgctgtccaaactggttgcatgga
aatgccaaaacattgaagtggtaagatcccaggtccctctgcagctgctgtaatgctttgtagtgagaatctgtaaatccacactcacttgctacattaa
ccaccagggttaccgaaccacggtacttctccagcgacaccagcttgcccctgata
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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