XB-CLONE-3189524
Clone Name: IMAGE:7850259
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Gene:
aldoa
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT388170 [+]
tgacccccgagcagaagaaggagctccatgacatcgccaaacgcattgtggccactggcaagggcatcctggcagccgatgagtccacaggcagcatcgc
taagcgcttgagttccatcggggcagagaacaccgaggagaaccgccgcttataccgccagctgctcttcaccgccgacgacagagtgaacccctgcatt
gggggcgtcatcaccttccacgagactctctaccacaagagcgacgatggcgtcgccttcaccgaagtcatcaagaaaaaagggggtgtcgtaggcatta
aggttgacaaaggtgtcgtccctctggctggaaccaacggtgaaaccacaactcaaggtctcgacggactgtccgagcgctgcgcccagtacaagaaaga
tggcgctgactttgccaagtggcgctgcgtgctcaagatctcagaacacactccctctcatctggccatcattgagaatgccaacgtattggctcgctat
gccagcatttgccaacagaacggaattgtccccattgtggagcccgaggttctccctgatggagaccatgacctgaagaggtgccagtatgtgacagaga
aggtcctggctgcagtgtacaaggctctcagcgaccaccacatctacctggaagggaccctgctgaaaccaaacatggttacccccgggcacgcctgcac
caagaagtacccccctcaggaggttgctatggcaacagtcaccgccctcaggcgcacagtgccccctgctgttggaggaatcaccttcctgtccggaggt
cagagtgagga
3' EST: DT388169 [+]ttttttgggtcagaaactgctttattgagggtttgttcagcagtgacacatagaacagtgactcagagccgagaatacagatacaggaggtgcacgtagc
ccggagacagacagtcgctctctctcacactcacacacgtcattcacgccctctgctggactgtccaggaatcaccatagcaacgctcagtccgcagtct
ctctctccagcaaggggaatggcttagctaatgttccgcaggcgggtctctacattggggaggggctaaccgctctgcaggggaggagcccctcactagg
caaatggaagcggccaccacagtgactaggaggagtaggaagtctagtaggcgtggtttgatacgaagagagactcgccggcagcagctcccgcctctcc
gctggctacgtatttgccttggcatgccaggctgttggccagggcacgcttgacgtactcctcctgggccttcttggtgttttccttcttgcccccccag
gctttgagggcggaggcctgaagggcacggccgtaggagaaggtcaggggccagggtttgatcagggggcactggttgatggcattgaggtgcacggtgg
cctcttcctcactctgacctccggacaggaaggtgattcctccaacagcagggggcactgtgcgcctgagggcggtgactgttgccatagcaacctcctg
aggggggtacttcttggtgcaggcgtgcccgggggtaaccatgtttggtttcagcagggtcccttccaggtagatgtggtggtcgctgagagccttgtac
actgcagccaggaccttctctgtcacatactggcacctcttcaggtcatggtctccatcagggagaacctcgggctcccaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: