XB-CLONE-3189482
Clone Name: IMAGE:7850167
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Gene:
pnpla6
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT388198 [+]
ctgggccatatcattattagaaagtggcatccagcactggatcataccattattatagagtagcatctaccactgggccatatcattattagaaagtggc
atccagcactggatcataccatttattattgagtggcatctatctgtgggtcatattattgccattagtcatgccattattatcgagtacatctttcttc
ggttcctaccattattacagagtgttgtccagcactggaccataccattaatatagagcagtgtttgtaactgggtcataccattaatacagagtagcat
ccagcactgagtcatatgattagtatagagtagcacccagaactgggtcataccattattataaagtgacaaccatcactggatcatgctattaatatag
agtaacattcatcactggatcatgtaattaatatagaatagcatccagaactggatcataccattaatatagagtaacattcatcaatggatcatgtaat
taatatagaatagcatccagaactggatcataccattaatatagagtaacattcatcaatggatcataccattgctatagagtagcatccatcaatggat
cataccattaatatagagtaacattcatcaatggatcataccattaatatagagtagcatccagcactgggccataccattaatgtagagtagcaaccag
cagtgggtgataccattattatagtgttacatccagcactgggccataccattattatagagttacatccagcactgggccataccattattatagagtt
acatccagcactgggccataccattattatagagttacatccagcactgggccataccattattatagagttacatccagcactgggccataccattatt
atagagttacatccagcactgggccatacccattatata
3' EST: DT388197 [+]ggtgtcaacgccttttattgtagaaaacctgttagtacatgagcagtcgccactgtttcctaaatgcaccccaaggcaagatgccccactggcaataagg
tcgggggcaggtagtgggggcatcagggccaggcagcagtggcagcagcaagaaactcaatgttccagtgaaacccggtgcagcagaagccccagcagca
atgggaggagtcactgagccaaagcttggtgctgattgggtggagttggccgattatggcacagaggctcatgggaaggggatgattgtcagcctggggc
cctccagcttcatactaacaagtgaggagatacaggttcaaccttagtattaagtcccaccagggagtagccacgccccacagacccccccccaatgcca
ggctgctgggagtccggcccagaccagggacattcaagtcacaaaacaaacaaacaaaaaaatcataattttataataaaaatgttaaagcaaaaacgta
tttaacgttactttggggatccagcgggggaggagagcagcttactgggagttaggggccctggggaaggataaagtgactggggccccccgtgctacag
tgccatgctgtaatgtagaatgtaacaaacccacagcattaaccgtataataaccctaatgccccctttattggtagggccagagccagatatagccccc
cccccggctatggggtaaaagcaggtatcagcggggccacagtgactaattccagtataatcccataagcagtgtcacgctgcacaggtaataac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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