XB-CLONE-3189386
Clone Name: IMAGE:7850271
|
Gene:
MGC147475
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT388262 [+]
ccgaggttttttttgagattggtacagagactttaatcatatccttcgctgcttcagcaaacacaaaggtaacaaaagcttcttctgggctgctgtgtta
ttgcactaaggcatacggggttatattattgtagcccttggcttgtgcattgagagaggccaaggcactcatgtaaaaaagcatggatgggtgctcggct
gcacccacaggtctgttaaacaataatctggcgttgagcgcgctgggaaacccctgaggtttggggacactgcagcccagtagatatgccctccgtgtca
gtcaagatatattttagttctggtatataaaagagaagaatactgtgagttggcaatgccagagcagtggtatttcctgtagggtttctgttgaggagta
actacaaggtaaggctgctgctactttctccatttgttcttgcagaaatctttaactcactggtggaagcaacctgctgcgttcagtctttgcatttctc
ctactcaaacccactgtaaccctcctcatccagaaatcacttccacatagttggcggggtaaagtcctacttgtcctttatctgtcactcctttgcacca
gccttgctcatcctcatcctcaatctttgtgagttcatcacctgatttaaaactgagctcgtcagattcctgccctgaataatcgtatacggctcgcacg
cgtactccaggaacattgattggagctttattttggctgctttctgtaggcgttacactgtataacttcaccttgtctgtctctttcactttcttctttt
tcacaatttgttgctctgtgtccggagtccactccttcagctgaggccagttcatgatcatcccangcccatgnnctgtc
3' EST: DT388261 [+]gaagtctatgcaggaagaagccgggcagagccatgtctgtgatttacagcgaggttgctgcggatgagaacaccaccgacagcttctggatgcccaataa
ctatggtagcaccgtgaagcgaatggaagatggctaccggctttgtgatgaaatcgtagcatgtttccaagacagagccaagatcgagcgtcagtatgct
ctacagatggaagaatggacccgcaagtggaaacctctggtagacagcagccctatgtatggttctctcctgcgcgcctggcaggcctttatgagtgcca
cagaacggctcagtgagctacatacacagatccagaagacattggtagcagaggatgcagagaagatacgcacgtggcagaaggatacatatcatcgcaa
gatatttggagggttcaaggagtcctgtgagattgagaatgggttccacaaggcacaaaagccatgggccaagaagttaaagaaggtggaaaagtccaag
gcaatttaccacaaagcctgtaagaaggagcacttggtcagtgtccgtgagaacagcagcaaaataaaccccgaacttactcaagagaagctgaagaaac
tcgccgaggatcatgagaaatgcacacaggacaaagaaaaggtaaagcagaggtatgagaagtccctacaggaactgaataagtataaccccaagtacat
ggaagaaatggaaacggtgtttgatcaaagtcagcaacaggagcagaagaagattctctttctcaaacaggtcctggagtctatgcacaaacatctggat
atcaccactaatgagagcgcaaagaaagtgtatagtgatttaaatgatgcgattgtcggggtaatgacc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: