XB-CLONE-3189374
Clone Name: IMAGE:7850081
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Gene:
coro1c
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT388270
[+]
ccaattgcactacatgaaatggacactagcaatggagttttgctaccattttatgatccagacacaagtatcatttacttgtgtggaaagggtgatagca
gtattcggtattttgaaatcacagatgagtctccatatgttcactacctcaacacattcagcagtaaggaaccacagcgagggatgggctatatgccaaa
gagagggttggatgtcaacaagtgtgaaattgccaggttctacaaattgcatgagaggaagtgcgagccaatcatcatgactgttcccaggaagtctgac
cttttccaagatgatctttaccctgatactgctggaccagaggctcctatagaggcagaggactggtttgatgggaaaaacgcagaccccgtattgattt
ccctaaagcaggggtatgtgccaaccaaaggcagagaccttaatgtggttaagaagaatatccttggcagcaagccagcagcccataaaaaggctgaacc
agccagcacccccaaaaagatgactgaaactccccatccagcaagtgatggcaagatggatgacgtcatgaaggaacttcggtcaatgaaagagaccatt
cggtaccaaggggaaaggattgccaagctagaacaattagttgccagtctcaacaccaatgatgagtcagaagaatgagcagtccatattcctgaataag
ctggaggatcccactgccagaacagattcccttcacattcctccaggcccaaatgatcagcattccgagaaaacagaccattcccttt
3' EST: DT388269
[+]gccgtcattatgcaagagaaccatttaggattacaggattagcaatttcccttgctagtcaaggtaatgctatctttttaaagtatggttatgctttatt
caacagcaagcaaatgctcttgtttctgtacagaacatcaaataagtcatgcacctggaggtcaccagtctcctgcaccaagccatcaaaaaggaatttc
ctttgtcattcaggagaattacacaataataaatcttgatttgtgggtggccaatatccctccttgcctggtgacctcaccatacaaatggacgtttctg
tgtatgatcagctttaacgtttcagacagaagtctaacatgtcaacacaaaccgcttcccaggctgtgaatgtctgactacacatatcgcagtaaagggg
tagcatatcgggatcagtctacattggaccatccatacttataacatgtgcaaaactcagtttttgtgaatggagacttttcctaaagagcagctggaga
tccattcattagcatgaatggaagctactgtaatacgagttggtactatggattttctgattttattaaaggcctgaaagttagaagaaacacatggaaa
aaccttaagcaaattaatttgtgcagcctgacattttgcaaagccactagagctaaaatcataaatcagtgttttagtctctagtggcacacaagcacga
ctgtaaaatgtgcaccaatcaaatgaatattttatctgttttaaagaggacaaaaccaccttaaaatcggaaagcaatgtggaatttgtgctggcttatg
catcatttaaaggtttcagtattgtgactctagnaatgccaagtctcagccctctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: