XB-CLONE-3189368
Clone Name: IMAGE:7850177
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Gene:
capza2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT388274 [+]
atcagttcactccagtaaaaattgatggctatgatgaacaggtattaataacagaacatggtgaccttggcaatgggaggtttttggacccaaaaagtaa
gctttcttttaagtttgatcacctaagaaaagaggcaagtgatcctaggccttccgatggagaaaatgcaattgaatcatggaggaacacagtggacact
gctgtaagatcctatgtgaaagaacattatccaaatggagtttgcactgtgtatggtaaaaccattgatggacaacaaaccattattgcatgtatagaga
gccatcagtttcaagctaaaaacttctggaatggacgttggaggtcagaatggaaatttacaataacgccatcaactacaaaagtgttcggcattttaaa
aattcaggttcattattatgaagatggtaatgtccagcttgtgagccataaagacatagaagagtcattaactgtttctaatgaggtccagactgctaag
gagttcattaaaattgtagaggatgctgaaaatgagtaccagactgcaatcagtgaaaattaccagactatgtctgacactacttttaaagccttacgtc
ggcagctgccagtgacacgcaccaagatcgactggaacaagattcttagttacaaaattggaaaagaaatgcaaaatgcctaggataaacattgcatggc
tgaaacatttttagtgtttttgcatcanaattattgcaaaaaagtatttggatctgccaaattgcagcatggccttttgcttaaaaaaaggaaattactg
caattaanataatttggctacagcacaaaattaac
3' EST: DT388273 [+]gatagaaaatctgcattttaatgtaacactgaattttagtacattttaactgcagaaaaaaaataaactgacagacatgatgagtacaggattacatata
tattacagtttgccttgttataatacattggaggctgtataataagattaagaaattaagggcaaatggatttcaagccaatttacattgttatcacagg
aacaaagcggtacatattatcaagcaatggctgacaaaaatccccaaatagtattagagctaggtctctctcttgacagcaggttcatacagttatcaaa
attaacagatttaacttttaaccattgtgccaaagcagcttctaacctgagccatctgcttttcatgttttgattagactgtttaggtgactaaataata
aaactgatggcacacaaggtgctttttcagattgacctgaatgggatatgcctaacagagccagtaccaccattaacagtcctctcagtactggcgctat
ctcattcaactcaatatgaggaggcaggggacatatctgggtcattttcaaatggttggacagtgctccatgtgccatcagactaaaagtgctggtttta
catatgccagggcagaggaagtcccccaaaaaatacattagggaaaatctctggttgtcacttaaagaaacctatcaaaatgagagcttcatacccgcct
gacacccaaaaagctgaaaccagacttttgtggcagatatgatctctcctctcacttacaaaatcatacatttggtttgctccatacctttacagtctct
tatgtaaaatcaaagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
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