XB-CLONE-3189356
Clone Name: IMAGE:7850369
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Gene:
aldoa
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT388282 [+]
ctcaccagtacccagcactgacccccgagcagaagaaggagctccatgacatcgccaaacgcattgtggccactggcaagggcatcctggcagccgatga
gtccacaggcagcatcgctaagcgcttgagttccatcggggcagagaacaccgaggagaaccgccgcttataccgccagctgctcttcaccgccgacgac
agagtgaacccctgcattgggggcgtcatcaccttccacgagactctctaccacaagagcgacgatggcgtcgccttcaccgaagtcatcaagaaaaaag
ggggtgtcgtaggcattaaggttgacaaaggtgtcgtccctctggctggaaccaacggtgaaaccacaactcaaggtctcgacggactgtccgagcgctg
cgcccagtacaagaaagatggcgctgactttgccaagtggcgctgcgtgctcaagatctcagaacacactccctctcatctggccatcattgagaatgcc
aacgtattggctcgctatgccagcatttgccaacagaacggaattgtccccattgtggagcccgaggttctccctgatggagaccatgacctgaagaggt
gccagtatgtgacagagaaggtcctggctgcagtgtacaaggctctcagcgaccaccacatctacctggaagggaccctgctgaaaccaaacatggttac
ccccgggcacgcctgcaccaagaagtacccccctcaggaggttgctatggcaacagtcaccgccctcaggcgcacagtgccccctgctgttggaggaatc
accttcctgtccggaggtcagagtgaggaagaggccaccgtgcacctcaatgccatcaaccagtgccccctg
3' EST: DT388281 [+]tttttttgagtgggtcagaaactgctttattgagggtttgttcagcagtgacacatagaacagtgactcagagccgagaatacagatacaggaggtgcac
gtagcccggagacagacagtcgctctctctcacactcacacacgtcattcacgccctctgctggactgtccaggaatcaccatagcaacgctcagtccgc
agtctctctctccagcaaggggaatggcttagctaatgttccgcaggcgggtctctacattggggaggggctaaccgctctgcaggggaggagcccctca
ctaggcaaatggaagcggccaccacagtgactaggaggagtaggaagtctagtaggcgtggtttgatacgaagagagactcgccggcagcagctcccgcc
tctccgctggctacgtatttgccttggcatgccaggctgttggccagggcacgcttgacgtactcctcctgggccttcttggtgttttccttcttgcccc
cccaggctttgagggcggaggcctgaagggcacggccgtaggagaaggtcaggggccagggtttgatcagggggcactggttgatggcattgaggtgcac
ggtggcctcttcctcactctgacctccggacaggaaggtgattcctccaacagcagggggcactgtgcgcctgagggcggtgactgttgccatagcaacc
tcctgaggggggtacttcttggtgcaggcgtgcccggnggtaaccatgtttggtttcagcagggtcccttccaggtagatgtggtggtcgctgagagcct
tgtacactgcagccaggaccttctctgtcacatactggcacctcttcaggtcatggtctccatcagggagaacctcgggctcccaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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