XB-CLONE-3189284
Clone Name: IMAGE:7850089
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Gene:
rpl7l1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT388330 [+]
agagaatctgctgaagaaacggaaaaagtatctggggctgaaagcagccgaggccaagagagcgttggaggagaagcgaaagacacagcctgggaagcaa
atcaagttcaagcgtttggaaaccttcgtacgaaactctcacaggaaactgagggacgacgctcggctgaagagaatgaagatttatcagaagaagattc
ctggcgtcgccacccagtcgctggcgtttgtggttcgtattacaaaagtcgatggagtcagtaacgcggttctggacgctctaaagacccttcgcttggg
gaagatttttgcaggagcttttgtgaagttgactccctcatccctcaacctgattcgagttgtagaaccgttcccaaatctgaaatcaatcagagagttg
gtcttaaaaaggggccatttcagaagggacggccggcgagtggctctgactgacaacaacgtgattgaggagcaactggggaagtttgggatcatctgtt
tggaggacgtgatccatgagttatacacaacgggggaacatttcccagaagtcaacaggttcctgtgccccttccgcctctcggtgtcgcgccatgcagc
cgtcaatcggaagggctacctgagcgaggtgggcgaccccggccacaggggctccggcattaaccagctgatccgccagctgaactagagcccctgctct
cagattgctgcgttccactcacggctgtctcttcacctacagagcgtctctggccgccccctggggcacaggaacatcatgtgactgatcccttagccaa
tggtgttggagagggcagtggataggggacacagggttcctgtacccc
3' EST: DT388329 [+]cttcagaaaatagttttattaaaaatgaaaagttgcaaaacaataaattgagggacttccagcgagagggagcgggtgtcacgcgggcaggttgggccca
actgtgtggcccttggggtacaggaaccctgtgtcccctatccactgccctctccaacaccattggctaagggatcagtcacatgatgttcctgtgcccc
agggggcggccagagacgctctgtaggtgaagagacagccgtgagtggaacgcagcaatctgagagcaggggctctagttcagctggcggatcagctggt
taatgccggagcccctgtggccggggtcgcccacctcgctcaggtagcccttccgattgacggctgcatggcgcgacaccgagaggcggaaggggcacag
gaacctgttgacttctgggaaatgttcccccgttgtgtataactcatggatcacgtcctccaaacagatgatcccaaacttccccagttgctcctcaatc
acgttgttgtcagtcagagccactcgccggccgtcccttctgaaatggcccctttttaagaccaactctctgattgatttcagatttgggaacggttcta
caactcgaatcaggttgagggatgagggagtcaacttcacaaaagctcctgcaaaaatcttccccaagcgaagggtctttagagcgtccagaaccgcgtt
actgactccatcgacttttgtaatacgaaccacaaacgccagcgactgggtggcga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: