XB-CLONE-3188959
Clone Name: IMAGE:7850310
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Gene:
arl6ip5
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT388546 [+]
tcccatcgattcgttttttcgccattcagaatgtcagtttattaatattaaatgcttgttttccccagatacaatgctcccatacacaactggggaaacg
gcacgattactgaacatgcattactatcagtcaggaatgtcggtgattctctggtctggaacagcagtgcaaaaactctgaatagtcaaacagaagatgc
attttatgttgcggggtgagcctggacgtatgttgtgaagatataaaaagtcgaatatcatccaaaccggttataaatgtatttaaaaatctgagccgtc
aatcatacatttcctgccccgcctctatgccgaaggcatagaggcggggcaggcaatacatgattgccagctcagacttccaaccacaattactccaact
ttgcattcagcacttccaagatattacctagatgacataggcataagattttggggtaatacaaagtttcccttaataacagtgtacacaaaatggcgcc
ggcctgctggctgtgactgtaaatcccaggactgaaggggaaaaaaaattaaatactttatacagtgtaagtaaagtttattttgctcaacgaacatgat
agaaaaggatttggaattattttttagggtaacaggtcccctttaattctaggggcaaacccaccaatcagctcattgcgctgccagcaactcaagggac
tatgcatataggcactgagttggttcttcttggtacataaggaggcacacgggggtggggtgaataatgtttctacccaaaaacatatttttagcaacca
aaaaaggagctggggaactcatagattaatatagttcttagttgttattagtgttgcactaatgctaccaaggcagcctgcatggtgctcgtgtaagcgg
ccatgttgaagcaattgacgctcagctgagcacaggcaggacaagaatgcataagaaatatatta
3' EST: DT388545 [+]agaaatgctgttttctttacatttccatgaggtacagcaactaggaggcagcgactccccctcttctcgttactcctccgctcctatgtaatcagtgcaa
gctcggtggtaccctactggaagcaacggaactaatgctgaggcgtcatgcctgtcttttgttggttaaatctcccagcatcctcagccaacatcatgga
gctgtggtttccaaagggtaggaaccctgtagttgctgtcaatgcttgtagcttgaaattttagttgcctgtcctcagcctccaaatctcacaattccct
gcacacgtgatgtcaataaggaaaggaacatcgcagtgcaatgcattgtgggttatgtagttcctgcatgctgtctgtaagctgtggagaatttgttaca
atttataacaccagtgttttagtccctcctcccctgccaggatttcaaatgatgcagaaagagaagaactgttttgcagctggatttcagcctataaaca
tgggatttatttaaacgtttttgactttacagtgaccggtatattaggggtttctgtgttgcgtggggctctttaacaaattttggttcggaagccagag
taaccctttaaattccatacttgggtcaaggactttcttgcaacgcctaaccactaagctgagacctgtaggcccctgtgggcggagccacattaacgga
atcattatcagttgtgtcatttgatttctattacctgttttgatgtagacatggtcccattaatatatttcttatgcattcttgtcctgcctgtgctcag
ctgagccgtcattgcttcaacatggccgcttacacgagcaccatgcaggctgccttggtagcattagtgcaaacactataacaactaagaactatattaa
tctatgagttccc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: