XB-CLONE-3188657
Clone Name: IMAGE:7850481
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Gene:
otud1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT388746 [+]
caggtttttttttaactcagtactaactgggaatgtaactaatcagcagaggatagaaatcgccattcttacaaaatcattaagtataaacaacagtaat
aaatactgaaccgcaatggggagaaaaaaacaaatgtaaacaaacattgtcatgtggcccataagtttcctacacacttggatctcaccagccaaaaagc
taaagtccagtcatttccactataacaaaaacaaatgaacacaaaatgttaacccttcatatagaaacaggtttgtaaataacaactgaaaatgtcttag
atataaataccacatgtccatttattattaactggcaagcggaagtctttgtccattaaaatgatgttacaaaaaaatagttaaggcagaggcatccaac
ctggggccctgcaactttagctaaactacaactcccagaagccttgacagatgctggaaatcgttattggggggggggtgaaggtaggacatccctgagt
taaggcatcaagtggtaaatgagaaggtttaaagtgcagcaaacttccactggtgagagtccaggtatatacacaacacagtgggcctggcaactggcag
tcagctggaacaaggcttggcacaccctgtgttgcatggactgcagaacgtattaggaaacactgactggtacccaaagagtcagagaggccattggtag
tgcctgtgatatttcctgctccagacactggcaatttgaggcctttggtgtaagggtgccaagtgtgtccctttagaactgtgagcctaacgcanagccc
cagctgcttgtaacgggcccggagtgcaaggccccagcaccgctgtctcctgtgtttatccatcatggctg
3' EST: DT388745 [+]atagggcgagaggcgcccagcagccccaggatatccatgcatggctcagcgacatggtgcaggagacgatccccaggcccgacaccatcgcggctcccat
agaagatcaatgctacgaaagtaacagcagcagcagcggcggcttttggcacccggaaagcccggagacgacggccgcacaatcgcaggccggtaacagg
gagcggttggccttacacctggccgagctggaaaagcaggacaagtacctgcgggaggagcagcgctatcgcttccatatcatcccggacggaaactgcc
tgtaccgggccgtgtgcaaggcgctatacggggagcaaagcctacacgccgagctgcgggagcgcaccgtgcactacgtggccgatcatctagacatttt
caacctcatcatcgagggcgacattggagaattcctcatcaacgccgcccaagatggggcctgggcgggatacccggagctcctggccatgagtcacatg
ctggaagtgaatatccgcctgaccaccgggggcaggcccgagtgccccaccgtctccaccatggttcatcggatcggaggcgaggatcccagccggccta
gtatctggctaagctggctcagcaacggccactacgacgcagtattcgagcagcccctgcccaacccagagtatgagcggtggtaccggcagaaccaagc
gcaaaggcagcgggacgaggaattggccaagtctatggccgtttccctctccaaaatgtacatcgagcaaaacccttgttcgtgaggcatcagcaaagct
ggcaaagcatggcaccctgggggcacccaggtaggcttccctgcaaaactggcacatgtggaaagcgcagccatgatggataaacacaggagacagcggt
gctggggccttgcactccggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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