XB-CLONE-3188628
Clone Name: IMAGE:7850627
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Gene:
ybx1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT388765 [+]
cgccggcctgagttaccatcaacaccccggggggaagttaaagcgagccaaccgccggcccgagtcaccacctccgaaagcagccaacttacaccatcaa
agacacaatgagcagcgaggttgaaacacaacagcagcagacagtcgcattggagggcaaggccggccaggaaccggcggccaccgtgggggagaagaag
gtcatcgcaaccaaggttttgggtacggtcaaatggtttaatgtgcgcaatggttacggcttcattaacaggaatgacaccaaggaagatgtgtttgtac
accaaactgccatcaagaagaataaccctaggaagtaccttcgcagtgtgggagatggtgaaactgttgagtttgatgtagtggagggtgaaaagggtgc
agaggcagctaatgtaactggtccagggggtgttccagtccaaggcagcaaatatgcagcagaccgtaatcatttcaggcgctatcagcgtcgcagaggt
cctccacgtaactaccagcaaaattaccaaaataatgaaagtggagagaagacagaagggaatgagagtgcacctgaaggagaggggggttcaaatcagc
aacgtccataccgcaggagacgtgttccaccatactacttgcggagaccatatgggcgcagaccacaatactcaaatgctcctattcagggagaaggtgc
agagggatcagatggtcaaggttcttcacaaggtgatcaaggtagacctgtgcgacagaatatgtacagaggcttcagaccacgatttcgcaggggacca
ccacggcag
3' EST: DT388764 [+]atgttgagacctttattaacagatgcttgcagtttgttgactcttgaaaaaaattaggtgtacgcttattacaaattaaaaatgaggttcttaaaaatct
tagtttgaccagataggaaacaatttaaaaacctttgaaaggtacattgagaaaaaatatgtttttaaaaaattttcattcccaaaaggagcttttagaa
aaaaaaacataaaaaccatctctgcatttacccatgcaggatgaagttatctggtcaagagcaaaaagcaagcacttaaggtcttcagttccaattcttt
gttcatttcttattgctggaatttctgtcttgacattttgatgacaaaaaaccggatggtggtagagttggtaagccggtatttactcagccccgccctg
ctcagcctcgggagcggacgtgttctcagctgatgtttcggctgccttggtctctttaccatcttgtggtttagggttttctgggcgtctgcgtctgtag
ttaaagttacggcgataccgacgttgaggtgcctgctgactctgggtttcatccccctgattttctttgtcctcttcatttccttcttctctgggttgtc
tctgccgtggtggtcccctgcgaaatcgtggtctgaagcctctgtacatattctgtcgcacaggtctaccttgatcaccttgtgaagaaccttgaccatc
tgatccctctgcaccttctccctgaataggagcatttgagtattgtggtctgcgcccatatggtctccgcaagtagtatggtggaacacgtctcctgcgg
tatggacgttgctgatttgaacccccctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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