XB-CLONE-3188518
Clone Name: IMAGE:7850637
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT388837 [+]
ccacatgcctttcggtaacacccataataactggaaattgaattattcctctgaggaggaatatcctgacctcaccaagcacaacaatcatatggccaag
gccctgtctgctgacatctacaagaagctcagggacaagcagacaccaagtggcttcaccttggatgatgtcattcagactggagttgacaacccaggcc
atcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcctatgaggtgttcaaggacctttttgatcccattattgaggatcgtcacggtgg
ttacaagccaactgacaaacacaagacagacctcaactttgccaaccttaagggaggtgatgaccttgacccaaactatgtgttgagcagccgtgtaagg
actggccgtagcattaagggatatgccctgcccccccactgcagccgtggggagcgtcgtgccattgaaaaacactccatcaaagctctcagcagtctgt
ctggggaattcaaagggaaatactaccctctcaaggatatgtcagatgcagagcagcagcagctgattgatgaccacttcctgtttgacaaaccagtgtc
ccctctgcttctggcctctggcatgggccgtgactggcctgatgcccgtggtatctggcacaatgaaaacaagaccttcctggtgtgggtaaatgaagag
gaccatctgagagtcatttccatgcagaaaggaggaaacatgaaagaagtcttcagacgcttctgtgagggacttcagaagattgaagagatctttaaac
aagcaggacaccccttcatgtggaatgaacatctgggttatgtccttacctgcccatcaaacttgggcacaggcctgagaggaggtgtc
3' EST: DT388836 [+]tagaggtgatattttggttttattgtctccaggcaatcattgggggggtttacaaaacaaatggtgaaaggatggtaaatgtgcaataatataatcttta
tcagagaattagatttatcttctcttttatgttgattttgaagtttttgttttcttgtgctggatcttcctactacatttctgagattatcccacccaca
tccattacttatgtattcccctgtagatggccatttttttgacctctaatgatcttaatggggtctcctgtcccctttgatggtggtaagggttagggtg
tttaggctctgtctctagcccctgtcatctacttctgtgctggcatcatatcatcaatggactgtcccttctcaagcttcttctccatctcaatcatgag
tttcacaccatccactaccatctggacttggtccacttcagagaagcccagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtca
acaccacctgtgcctctcttctgcagacgaagcctgcccagtatctcatcaaatttggggtgtttgctgaggttggggagcttggcatggacacctcctc
tcaggcctgtgcccaagtttgatgggcaggtaaggacataacccagatgttcattccacatgaaggggtgtcctgcttgtttaaagatctcttcaatctt
ctgaagtccctcacagaagcgtctgaagacttctttcatgtttcctcctttctgcatggaaatgactctcagatggtcctcttcatttacccacaccagg
aaggtcttgttttcattgtgccagataccacgggcatcaggccagtcacggcccatgccagaggccagaagcagaggggacactggtttgtcaaacag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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