XB-CLONE-3188506
Clone Name: IMAGE:7850447
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Gene:
aldoa
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT388845 [+]
ccgcttataccgccagctgctcttcaccgccgacgacagagtgaacccctgcattgggggcgtcatcaccttccacgagactctctaccacaagagcgac
gatggcgtcgccttcaccgaagtcatcaagaaaaaagggggtgtcgtaggcattaaggttgacaaaggtgtcgtccctctggctggaaccaacggtgaaa
ccacaactcaaggtctcgacggactgtccgagcgctgcgcccagtacaagaaagatggcgctgactttgccaagtggcgctgcgtgctcaagatctcaga
acacactccctctcatctggccatcattgagaatgccaacgtattggctcgctatgccagcatttgccaacagaacggaattgtccccattgtggagccc
gaggttctccctgatggagaccatgacctgaagaggtgccagtatgtgacagagaaggtcctggctgcagtgtacaaggctctcagcgaccaccacatct
acctggaagggaccctgctgaaaccaaacatggttacccccgggcacgcctgcaccaagaagtacccccctcaggaggttgctatggcaacagtcaccgc
cctcaggcgcacagtgccccctgctgttggaggaatcaccttcctgtccggaggtcagagtgaggaagaggccaccgtgcacctcaatgccatcaaccag
tgccccctgatcaaaccctggcccctgaccttctcctacggccgtgcccttcaggcctccgccctcaaagcctgggggggcaagaaggaaaacaccaaga
aggcccaggaggagtacgtcaagcgtgccctggccaacagcctggc
3' EST: DT388844 [+]ttgggtcagaaactgctttattgagggtttgttcagcagtgacacatagaacagtgactcagagccgagaatacagatacaggaggtgcacgtagcccgg
agacagacagtcgctctctctcacactcacacacgtcattcacgccctctgctggactgtccaggaatcaccatagcaacgctcagtccgcagtctctct
ctccagcaaggggaatggcttagctaatgttccgcaggcgggtctctacattggggaggggctaaccgctctgcaggggaggagcccctcactaggcaaa
tggaagcggccaccacagtgactaggaggagtaggaagtctagtaggcgtggtttgatacgaagagagactcgccggcagcagctcccgcctctccgctg
gctacgtatttgccttggcatgccaggctgttggccagggcacgcttgacgtactcctcctgggccttcttggtgttttccttcttgcccccccaggctt
tgagggcggaggcctgaagggcacggccgtaggagaaggtcaggggccagggtttgatcagggggcactggttgatggcattgaggtgcacggtggcctc
ttcctcactctgacctccggacaggaaggtgattcctccaacagcagggggcactgtgcgcctgagggcggtgactgttgccatagcaacctcctgaggg
gggtacttcttggtgcaggcgtgcccgggggtaaccatgtttggtttcagcagggtcccttccaggtagatgtggtggtcgctgagagccttgtacactg
cagccaggaccttctctgtcacatactggcacctcttcaggtcatggtctcca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: