XB-CLONE-3188207
Clone Name: IMAGE:7850665
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Gene:
psma6
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389042 [+]
cttctcacccgaagggcgtctctatcaagtcgaatatgcattcaaggcgatcaaccaggggggtcttacatcagtggctgtcagaggaaaagactgtgct
gttgttgttacccaaagaaaagtcccagacaagctgttggacagcaacacagtaacccatttattccggattactgaatccattggctgtgtaatgaccg
gaatgacagctgacagcaggtcgcaggtccagcgggcacgttatgaggctgcaaattggaagtacaaatacggctatgaaatcccagtagatatgctttg
taaaaggattgcggatatttctcaagtatacacacagaatgctgaaatgaggcccttaggatgttgcatgatcctaattggagtggatgaggagaatgga
ccacaagtctttaaatgtgatcctgcaggctactattgtggctttaaggctaccacagctggagtgaagcaaactgaggccaccagcttcctagaaaaga
aagtaaagaagaaattggactggacttatgagcagactcttgagacagccatttcttgtctctccacggtactgtctattgatttcaaaccttcagaact
ggaggtgggagtcgtcacagtcgaggatccaaaattcagagttcttacagaagcagagattgatgtccaccttgttgcactagctgagcgtgactagtca
tcctactttcacctctgcccagaaagctcttgcctggtaaaatcacctgtcttcataacactttttgttttgttctctacataaataanacagatttttt
tctttttggaaaa
3' EST: DT389041 [+]atagggcgagaggtttttccaaaagaaaaaaatctgttttatttatgtagagaacaaaacaaaaagtgttatgaagacaggtgattttaccaggcaagag
ctttctgggcagaggtgaaagtaggatgactagtcacgctcagctagtgcaacaaggtggacatcaatctctgcttctgtaagaactctgaattttggat
cctcgactgtgacgactcccacctccagttctgaaggtttgaaatcaatagacagtaccgtggagagacaagaaatggctgtctcaagagtctgctcata
agtccagtccaatttcttctttactttcttttctaggaagctggtggcctcagtttgcttcactccagctgtggtagccttaaagccacaatagtagcct
gcaggatcacatttaaagacttgtggtccattctcctcatccactccaattaggatcatgcaacatcctaagggcctcatttcagcattctgtgtgtata
cttgagaaatatccgcaatccttttacaaagcatatctactgggatttcatagccgtatttgtacttccaatttgcagcctcataacgtgcccgctggac
ctgcgacctgctgtcagctgtcattccggtcattacacagccaatggattcagtaatccggaataaatgggttactgtgttgctgtccaacagcttgtct
gggacttttctttgggtaacaacaacagcacagtcttttcctctgacagccactgatgtaagacccccctggttgatcgccttgaatgcatattcgactt
gatagagacgcccttcgggtgagaag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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