XB-CLONE-3188182
Clone Name: IMAGE:7850668
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389058 [+]
ccgaggccacaatgggaaaggaaaagactcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaaccggacatcttatctacaa
atgtggtggtatcgacaagagaaccatcgaaaagtttgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttggacaaactg
aaggccgagcgtgaacgtggtatcaccattgacatctccctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccaggacacagag
acttcatcaagaacatgatcactggtacctctcaggctgactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtatctcaaagaa
cggacaaactcgtgagcatgccctccttgcctacactctgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaacccccatacagc
cagaaaagatatgaggaaatcgttaaggaagtcagcacatacatcaagaagattggttacaaccctgacactgttgcctttgtacctatttctggatgga
acggtgacaacatgcttgagcccagcgccaatatgccttggtttaaggggtggaaaatctcacgtaaagagggatctggcagcggaactaccctgctgga
agctcttgactgcattttgccaccaagtcgcccaactgataagcctctgcgtctgcctctgcaggatgtctacaaaattggcggtattggtactgtacca
gttggtcgtgtggagactggtgtccttaagcaggcatggtggttacttttgcccctgntaatgtaacaactgaa
3' EST: DT389057 [+]aactttttttttaatggtctcaatttggtgacagattttggtcaagttgcttccatttagaaattactggatttaaaacactaataacttaaaaactgcc
acaaacaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttgactattaaactctgatggccaatt
gatagaaagagttctgagaccaatcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgcctttctgtgctttctgagcagact
ttgtgactttgccagctccagcagccttcttatcgaccgccttgatgactcctacagcaacagtctgcctcatgtcacggacagcaaaacgaccaagagg
ggggtagtcagagaagctctccacgcacataggctttcctgggatcatgtcaacaatggcagcatcaccagacttcaggaacttgggattgtcttccagt
ttcttaccagaacggcgatcaatcttttccttcagctcagcaaacttgcaagcaatgtgagctgtgtggcaatccaacacaggggcatatccagcaccaa
tctggcctgggtggttcaggatgataacctgtgctgtaaaggtaccagcttccattggtgggtcattcttgctgtcaccagcaacgttgccacgacggac
gtccttcacagaaacgtttttcacgttaaagccaacattgtcaccgggcatggcctcgctaagggcttcatggtgcatttcaacagatttaacttcagtt
gttacattaacaggggcaaaagtaaccaccatgcctggcttaaggacaccag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: