XB-CLONE-3188077
Clone Name: IMAGE:7850582
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Gene:
arrdc2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389127 [+]
acagggaatacagggaatatctggaaatttcccactgaagtattttttcaacagcccgagggtcatgagtaatcccatctcagagtttaaagtcctgact
tatatgtaaggtcacttaatagaatgaaaaggcactgtagcaccaaattccgctttcaaagcaatcctctttcattaatactgaaaaaatataaggtgat
tttacagattgaataaataataaatcaatgtgcttgaaatataaacttacaaagcttacactattacaattttataaagttcactttcaactcataatta
atatgtatcttttttttttctcccttaagaggacttaaaaaaaaaaaaaaaaggtttgctttaaggctactgctgccatggcatggaataacaaattaag
gcagatactgtgtgtatagctattccataggtcaagcagatgtcataataaaagtaaacttttttcttttttagatcccagttcaatctttggttgtatg
caggcactgtagaaattacagggggaaaatatctgctctacaatgtttaaagaatgaaaaaggcagaacattctttagctcagcacctgctaagcaaact
atgaaccagactgactgtactgtaaacattaactttggttgtgtgaaatgcttagcccttaacagtttggtttctgtgctctgggcaagcaaagcctgca
acatgctgggtgatgtcaggcatttgacaatttcagctagaatgtacaattgtctagaanaaaataaaaatgctgtactattacagttttagtaacatct
cataacagcttagttttttctttagaagtgcagaaagcctgtttttccccagagctc
3' EST: DT389126 [+]ttttttgacagatctgttattcaaggatcattttgtttctagctggtttttgcttcattgatcctgcttttaatatagcttgcacaagttttattcattt
cttcggtgcgcgttgcttagaaagagcaggatgatctttgacaaagtgaggaaattccttgttgagcttgactctgccggcttggatagccccttagtgt
ttcgcagcggggacacagtgacggggaaggtgttgttggaactggtggctgaggctaaagtggagtcggtgaagctgcacgcagggggttatgctaaggc
acactggactgagtcgcgcagtgccggctctagcactgcttatactcagaactatagcgatgaggtggagtacattaacttgcgggagaccctactgcaa
acagataatggcacacttaccatgctgccagcaggaaggcatgaatttcccttcagctttcaactgcctgagaccctagtaacgtcatttgaaggaaagc
atggaagtgttcgctattgggtaaaggcaaagttgcatcgtccatggtgtacagtgaagaaagtcaagaaggaattcactgtaatagaacctattgatat
caacactccagatctcttggctccacaagctggcagcaaggagaagattgcacatgcctggtactgcaacctggggcatgtctcagtcactgcaaagata
gaccgtaaaggctatactccaggtgaagtgattccgatctttgctgaaattgacaactgcactacccgtgctgtgatacccaaggcagctattatccagt
cgcaggcattcgttgctcgtggtaccatgaagcaaaagaaatctgtggtagccactttggcaggggatgcagtgcctgcaggaaaaagggaaacctggca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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