XB-CLONE-3187938
Clone Name: IMAGE:7850690
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Gene:
mustn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389219 [+]
ccacagggtgccccagttaagaagaagaggcctgtgatgaaggaggaagaccttaaaggagcaaaacacaagctggcccaccaggtcccaatcaaatcca
aaacataccaggttatgcaggagtgcgaacaatctggaacaacagccccttctgtgttcagccagaacaggacgggcggagaaacagcctttgacaagcc
aaaggaaggcccagcgaaaagtgtctttggttaatggcaaaagaacattcaaataactcggctgcccagcaagtcatttttgttcccaccttctactcat
tgtgctgcgcacatcatgcagagtcttacactatatccgcgccagtcatttcactgtctgttatctgtttctaagttactgtaagactttatttcattgc
tcctatggtcgggctgtgctgcattatctgatatactttcagtagagacccatttatatgttttctaaactggctcttcagttattaagtcaacaaagga
gccaaatatgattttttagatttcctatatataaaatggaggaagtgaatacagaatttgtattttctaaaatccaaacccttacaggtccttaggcttt
ggctggaccaaatcagaacacctcttttcgtcatgtgactttactgggctggggacctaaacacagtcatatcacctactccccatcatcagggtgatat
ggttcagccctggcatttttcctatgtcaacatattaaggtggattcaggactagaccatttcctgcctggtgagggatcacctgctttaattgttgcat
tanatgtaaaattaggacttttccc
3' EST: DT389218 [+]gttccaagtttaattgtaaaaccaggaaagtttaaaaaaaacaaaccatataagaacatagcgacacaagaatgatcagggctaatacacaacacacatt
tacttctcagtgatgctgtgactgggtgctctgttctacaaggtttcatttaatacaccctgacactatctagcaccccgagcagaagcctagtgtgatg
ccagtggcactgagttactacaaggactaagcctgaatgtgtggaagggtgaataggttggggccttatacactgaccaatcatagaactaactggaaca
cagtaaatctctcttgccaagtcgagctgtcgctgctggtcaatagaccaatcataactaattgctcctacaaggtcattcacttattgaatagaaggct
acaaggtcaactttctctttattctgtcttctaatatcattactggctacttgagatatggacgccgtttttattctacagttggagcctataaaggttg
ctcagattatcagcccttagtttgttagggatgagtacctaacgggaaaagtcctaattttacatttaatgcaacaattaaagcaggtgatccctcacca
ggcaggaaatggtctagtcctgaatccaccttaatatgttgacataggaaaaatgccagggctgaaccatatcaccctgatgatggggagtaggtgatat
gactgtgtttaggtccccagcccagtaaagtcacatgacgaanagaggtgttctgatttggtccagccaaagcctaaggacctgtaagggtttggatttt
agaaaatacaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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