XB-CLONE-3187716
Clone Name: IMAGE:7850614
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Gene:
ybx1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389366 [+]
ttttatgttgagacctttattaacagatgcttgcagtttgttgactcttgaaaaaaattaggtgtacgcttattacaaattaaaaatgaggttcttaaaa
atcttagtttgaccagataggaaacaatttaaaaacctttgaaaggtacattgagaaaaaatatgtttttaaaaaattttcattcccaaaaggagctttt
agaaaaaaaaacataaaaaccatctctgcatttacccatgcaggatgaagttatctggtcaagagcaaaaagcaagcacttaaggtcttcagttccaatt
ctttgttcatttcttattgctggaatttctgtcttgacattttgatgacaaaaaaccggatggtggtagagttggtaagccggtatttactcagccccgc
cctgctcagcctcgggagcggacgtgttctcagctgatgtttcggctgccttggtctctttaccatcttgtggtttagggttttctgggcgtctgcgtct
gtagttaaagttacggcgataccgacgttgaggtgcctgctgactctgggtttcatccccctgattttctttgtcctcttcatttccttcttctctgggt
tgtctctgccgtggtggtcccctgcgaaatcgtggtctgaagcctctgtacatattctgtcgcacaggtctaccttgatcaccttgtgaagaaccttgac
catctgatccctctgcaccttctccctgaataggagcatttgagtattgtggtctgcgcccatatggtctccgcaagtagtatggtggaacacgtctcct
gcggtatggacgttgctgatttgaacccccctctccttcaggtgcactcatttccttctgtc
3' EST: DT389365 [+]ccccgccggcctgagttaccatcaacaccccggggggaagttaaagcgagccaaccgccggcccgagtcaccacctccgaaagcagccaacttacaccat
caaagacacaatgagcagcgaggttgaaacacaacagcagcagacagtcgcattggagggcaaggccggccaggaaccggcggccaccgtgggggagaag
aaggtcatcgcaaccaaggttttgggtacggtcaaatggtttaatgtgcgcaatggttacggcttcattaacaggaatgacaccaaggaagatgtgtttg
tacaccaaactgccatcaagaagaataaccctaggaagtaccttcgcagtgtgggagatggtgaaactgttgagtttgatgtagtggagggtgaaaaggg
tgcagaggcagctaatgtaactggtccagggggtgttccagtccaaggcagcaaatatgcagcagaccgtaatcatttcaggcgctatcagcgtcgcaga
ggtcctccacgtaactaccagcaaaattaccaaaataatgaaagtggagagaagacagaagggaatgagtgcacctgaaggagaggggggttcaaatcag
caacgtccataccgcaggagacgtgttccaccatactacttgcggagaccatatgggcgcagaccacaatactcaaatgctcctattcagggagaaggtg
cagagggatcagatggtcaaggttcttcacaaggtgatcaaggtagacctgtgcgacagaatatgtacagaggcttcagaccacgatttcgcaggggacc
acctacgcagagacaacccagagaagagggaaatgagaggacaaagaaaatcaggggga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: